Detailed information of TA system TA031429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2056545..2057074
• Replicon: chromosome
• Accession: NZ_AP017922
• Organism: Staphylococcus aureus strain JP02758

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JP49775_RS10290
• Protein ID: WP_000621175.1
• Coordinates: 2056545..2056907 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JP49775_RS10295
• Protein ID: WP_000948331.1
• Coordinates: 2056904..2057074 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JP49775_RS10270	2053523..2054293	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
JP49775_RS10275	2054268..2054747	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
JP49775_RS10280	2054749..2055075	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JP49775_RS10285	2055194..2056195	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JP49775_RS10290	2056545..2056907	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JP49775_RS10295	2056904..2057074	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JP49775_RS10300	2057159..2058307	-	1149	WP_029550314.1	alanine racemase	-
JP49775_RS10305	2058373..2058732	-	360	WP_029550315.1	holo-ACP synthase	-
JP49775_RS10310	2058736..2059227	-	492	WP_072435149.1	PH domain-containing protein	-
JP49775_RS10315	2059220..2060797	-	1578	WP_029550317.1	PH domain-containing protein	-
JP49775_RS10320	2060790..2061269	-	480	WP_001287081.1	hypothetical protein	-
JP49775_RS10325	2061478..2062038	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T31429 WP_000621175.1 NZ_AP017922:c2056907-2056545 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T31429 NZ_AP017922:c2056907-2056545 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT31429 WP_000948331.1 NZ_AP017922:c2057074-2056904 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT31429 NZ_AP017922:c2057074-2056904 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1