Detailed information of TA system TA031406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2855658..2856295
• Replicon: chromosome
• Accession: NZ_AP017901
• Organism: Mycobacterium tuberculosis strain NCGM946K2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: CPZ08_RS13850
• Protein ID: WP_003413180.1
• Coordinates: 2855658..2856053 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: CPZ08_RS13855
• Protein ID: WP_003413183.1
• Coordinates: 2856050..2856295 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ08_RS13805	2851061..2852272	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
CPZ08_RS13815	2852399..2853058	+	660	WP_003900846.1	LppA family lipoprotein	-
CPZ08_RS13820	2853055..2853717	+	663	WP_003900848.1	hypothetical protein	-
CPZ08_RS13825	2853714..2853992	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
CPZ08_RS13830	2854085..2854498	+	414	WP_003413164.1	PIN domain nuclease	-
CPZ08_RS13835	2854537..2854794	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
CPZ08_RS13840	2854791..2855168	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
CPZ08_RS13845	2855184..2855558	-	375	WP_003413177.1	hypothetical protein	-
CPZ08_RS13850	2855658..2856053	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
CPZ08_RS13855	2856050..2856295	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
CPZ08_RS13865	2856706..2857125	-	420	WP_003413190.1	A24 family peptidase	-
CPZ08_RS13870	2857137..2857946	-	810	WP_003413193.1	shikimate dehydrogenase	-
CPZ08_RS13875	2857943..2859196	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
CPZ08_RS13880	2859189..2859701	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T31406 WP_003413180.1 NZ_AP017901:c2856053-2855658 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T31406 NZ_AP017901:c2856053-2855658 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT31406 WP_003413183.1 NZ_AP017901:c2856295-2856050 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT31406 NZ_AP017901:c2856295-2856050 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31