Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2854537..2855168 Replicon chromosome
Accession NZ_AP017901
Organism Mycobacterium tuberculosis strain NCGM946K2

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag CPZ08_RS13840 Protein ID WP_003413174.1
Coordinates 2854791..2855168 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag CPZ08_RS13835 Protein ID WP_003413167.1
Coordinates 2854537..2854794 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ08_RS13800 2850451..2850765 + 315 WP_009937839.1 hypothetical protein -
CPZ08_RS13805 2851061..2852272 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CPZ08_RS13815 2852399..2853058 + 660 WP_003900846.1 LppA family lipoprotein -
CPZ08_RS13820 2853055..2853717 + 663 WP_003900848.1 hypothetical protein -
CPZ08_RS13825 2853714..2853992 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
CPZ08_RS13830 2854085..2854498 + 414 WP_003413164.1 PIN domain nuclease -
CPZ08_RS13835 2854537..2854794 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
CPZ08_RS13840 2854791..2855168 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
CPZ08_RS13845 2855184..2855558 - 375 WP_003413177.1 hypothetical protein -
CPZ08_RS13850 2855658..2856053 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
CPZ08_RS13855 2856050..2856295 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
CPZ08_RS13865 2856706..2857125 - 420 WP_003413190.1 A24 family peptidase -
CPZ08_RS13870 2857137..2857946 - 810 WP_003413193.1 shikimate dehydrogenase -
CPZ08_RS13875 2857943..2859196 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CPZ08_RS13880 2859189..2859701 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T31405 WP_003413174.1 NZ_AP017901:2854791-2855168 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T31405 NZ_AP017901:2854791-2855168 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT31405 WP_003413167.1 NZ_AP017901:2854537-2854794 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT31405 NZ_AP017901:2854537-2854794 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCGGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References