Detailed information of TA system TA031356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2251271..2251803
• Replicon: chromosome
• Accession: NZ_AP017898
• Organism: Sphingopyxis sp. FD7

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: SPYCA_RS10655
• Protein ID: WP_120220288.1
• Coordinates: 2251271..2251513 (+)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: SPYCA_RS10660
• Protein ID: WP_120222278.1
• Coordinates: 2251552..2251803 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPYCA_RS10640	2247016..2248233	-	1218	WP_120220282.1	MFS transporter	-
SPYCA_RS10645	2248425..2250248	+	1824	WP_120220284.1	cobaltochelatase subunit CobT	-
SPYCA_RS10650	2250478..2251158	+	681	WP_146625122.1	hypothetical protein	-
SPYCA_RS10655	2251271..2251513	+	243	WP_120220288.1	BrnT family toxin	Toxin
SPYCA_RS10660	2251552..2251803	+	252	WP_120222278.1	BrnA antitoxin family protein	Antitoxin
SPYCA_RS10665	2252348..2253994	+	1647	WP_120220290.1	acetolactate synthase large subunit	-
SPYCA_RS10670	2254106..2255539	+	1434	WP_172595041.1	aldehyde dehydrogenase family protein	-
SPYCA_RS10675	2255767..2256639	+	873	WP_120220292.1	UDP-2,3-diacylglucosamine diphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(3)-Ii	-	2220681..2343958	123277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-76)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (7-80)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 8798.20 Da
• Isoelectric Point: 7.3199

>T31356 WP_120220288.1 NZ_AP017898:2251271-2251513 [Sphingopyxis sp. FD7]
MILWDESKRQINLAKHGFDFADLSEEFFLSAQVIPAKGGRHMAIGRLDDGTIAVVFALLGTEGVSVISMRHASKKERGLL

Nucleotide

• Length: 243 bp

>T31356 NZ_AP017898:2251271-2251513 [Sphingopyxis sp. FD7]
ATGATCCTCTGGGACGAATCGAAGCGGCAGATCAATCTCGCCAAGCACGGGTTCGATTTCGCCGATTTGTCGGAAGAGTT
TTTCCTGTCGGCTCAGGTGATCCCGGCGAAGGGCGGTCGGCATATGGCGATCGGGCGCCTCGACGATGGAACGATTGCGG
TGGTGTTCGCCCTGCTCGGCACCGAAGGGGTGTCGGTGATCTCGATGCGCCACGCCAGCAAAAAGGAAAGAGGCCTGTTA
TGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8907.20 Da
• Isoelectric Point: 10.3860

>AT31356 WP_120222278.1 NZ_AP017898:2251552-2251803 [Sphingopyxis sp. FD7]
MIASDPDAPEATDEQLAQARPFADAFPALADKMRRNVGGRPKADKTKVAVSIRLDSEVLEAFKAGGPGWQSRMNKALRKV
AGI

Nucleotide

• Length: 252 bp

>AT31356 NZ_AP017898:2251552-2251803 [Sphingopyxis sp. FD7]
ATGATCGCCAGCGATCCCGACGCGCCCGAAGCGACCGACGAGCAACTGGCGCAGGCGCGCCCCTTTGCCGACGCCTTCCC
CGCGCTGGCGGACAAGATGCGCCGGAATGTCGGCGGACGGCCGAAAGCCGACAAGACCAAGGTCGCCGTCTCGATCCGGC
TCGACTCCGAAGTGCTCGAAGCCTTCAAGGCTGGCGGCCCCGGCTGGCAATCGCGCATGAACAAGGCGCTGCGCAAGGTC
GCGGGGATATGA