Detailed information of TA system TA031332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 43020..43579
• Replicon: plasmid pSCLO_4
• Accession: NZ_AP017658
• Organism: Sphingobium cloacae strain JCM 10874

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: SCLO_RS21600
• Protein ID: WP_066522399.1
• Coordinates: 43020..43313 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SCLO_RS21605
• Protein ID: WP_066522401.1
• Coordinates: 43301..43579 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCLO_RS21580	38167..39159	-	993	WP_123905556.1	serine/threonine protein phosphatase	-
SCLO_RS21585	39455..40219	+	765	WP_066522521.1	IS6 family transposase	-
SCLO_RS21590	40277..42709	+	2433	Protein_43	Tn3 family transposase	-
SCLO_RS21595	42706..42972	-	267	WP_083949265.1	XRE family transcriptional regulator	-
SCLO_RS21600	43020..43313	-	294	WP_066522399.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SCLO_RS21605	43301..43579	-	279	WP_066522401.1	CopG family ribbon-helix-helix protein	Antitoxin
SCLO_RS21610	43744..46635	-	2892	WP_016746447.1	Tn3 family transposase	-
SCLO_RS21615	46761..47381	+	621	WP_008832583.1	recombinase family protein	-
SCLO_RS21620	47378..47908	+	531	WP_007686150.1	GNAT family N-acetyltransferase	-
SCLO_RS21625	48158..48490	-	333	Protein_50	error-prone DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..108910	108910
	-	inside	IScluster/Tn	-	-	37313..52528	15215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10812.36 Da
• Isoelectric Point: 7.2017

>T31332 WP_066522399.1 NZ_AP017658:c43313-43020 [Sphingobium cloacae]
VPRLIWTPEALADVDRLHRWLKPKDADAASRAVAAIRAGVKILAQSPRVGRPAEDMDPEYREKLIDFGDSGYVVLYRLDG
ETVALLAVRHQKEAGYS

Nucleotide

• Length: 294 bp

>T31332 NZ_AP017658:c43313-43020 [Sphingobium cloacae]
GTGCCACGTCTGATCTGGACGCCGGAAGCACTGGCCGATGTTGACCGGCTCCATCGCTGGTTGAAGCCCAAGGACGCCGA
CGCGGCGAGCCGTGCGGTCGCGGCGATCCGGGCAGGGGTCAAGATTCTGGCGCAATCGCCGCGCGTCGGCCGACCTGCGG
AAGATATGGACCCTGAATATCGGGAAAAGCTGATCGACTTTGGAGATAGCGGCTATGTCGTCCTGTATCGCCTCGACGGC
GAGACGGTGGCGTTGCTGGCCGTTCGCCACCAAAAGGAGGCGGGCTATTCATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10660.02 Da
• Isoelectric Point: 6.0905

>AT31332 WP_066522401.1 NZ_AP017658:c43579-43301 [Sphingobium cloacae]
MAAATSIKLDDTLKGRVQHLADARRRSSHWIMREAIAQYVEREEKREAFKQDAIRAWEEYQRTGLHLTLEEADAWLAKLE
AGEDAELPKCHV

Nucleotide

• Length: 279 bp

>AT31332 NZ_AP017658:c43579-43301 [Sphingobium cloacae]
ATGGCAGCGGCAACATCTATCAAGCTGGACGATACCCTGAAAGGGCGCGTGCAGCACTTGGCCGACGCGCGCCGTCGCAG
CTCGCATTGGATCATGCGCGAAGCCATCGCGCAGTATGTCGAGCGCGAGGAAAAGCGCGAGGCGTTCAAGCAGGACGCGA
TACGGGCATGGGAGGAATATCAGCGGACGGGCTTGCATCTGACGCTTGAGGAAGCGGACGCCTGGTTGGCGAAGCTGGAA
GCGGGCGAGGATGCGGAGCTGCCTAAGTGCCACGTCTGA