Detailed information of TA system TA031327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 188216..189018
• Replicon: chromosome
• Accession: NZ_AP017655
• Organism: Sphingobium cloacae strain JCM 10874

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A1E1EY53
• Locus tag: SCLO_RS01020
• Protein ID: WP_066516491.1
• Coordinates: 188491..189018 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: SCLO_RS01015
• Protein ID: WP_066516583.1
• Coordinates: 188216..188494 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCLO_RS00995	184165..185688	+	1524	WP_066516489.1	IS21 family transposase	-
SCLO_RS01000	185678..186484	+	807	WP_037523679.1	IS21-like element helper ATPase IstB	-
SCLO_RS01005	186539..187030	+	492	Protein_199	TonB family protein	-
SCLO_RS01010	187190..187966	+	777	WP_066516490.1	LuxR family transcriptional regulator	-
SCLO_RS01015	188216..188494	+	279	WP_066516583.1	DUF1778 domain-containing protein	Antitoxin
SCLO_RS01020	188491..189018	+	528	WP_066516491.1	acetyltransferase	Toxin
SCLO_RS01025	189264..189719	+	456	WP_066516492.1	hypothetical protein	-
SCLO_RS01030	189716..193486	-	3771	WP_066516495.1	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	413..209272	208859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (7-89)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19052.63 Da
• Isoelectric Point: 7.0509

>T31327 WP_066516491.1 NZ_AP017655:188491-189018 [Sphingobium cloacae]
MTLPSWHEEAISKAHDRASFDCGDNDLNVYLQRYARQSHEAGGAKTFLAIDDTDNKTVLGFYSIAPGSMDHADTPELARR
GLARHEVPGFRLARLATDVRAQGKGLGGQLLGAIGRRCIRVAAEVGGVMLIIDAKNERAADWYASYGAIPLHDKPLTLIL
PFATLERELRAAGQL

Nucleotide

• Length: 528 bp

>T31327 NZ_AP017655:188491-189018 [Sphingobium cloacae]
ATGACGCTTCCTTCATGGCACGAGGAAGCGATTTCGAAAGCGCATGACCGGGCTTCATTCGACTGCGGCGACAATGATCT
GAACGTCTACTTGCAGCGTTATGCGCGGCAAAGCCACGAGGCAGGCGGCGCGAAGACGTTCCTCGCCATCGACGACACCG
ACAACAAGACGGTTCTCGGCTTTTACAGCATTGCTCCGGGCAGCATGGATCACGCGGACACGCCAGAACTGGCGAGGCGA
GGACTCGCCAGGCATGAGGTGCCGGGCTTCCGGCTCGCGCGGCTTGCGACGGATGTGCGTGCGCAGGGGAAAGGACTCGG
GGGTCAGCTCCTGGGCGCGATCGGGCGCAGATGCATTCGCGTCGCGGCCGAGGTCGGCGGGGTTATGCTGATCATCGACG
CCAAAAACGAGCGCGCCGCCGACTGGTATGCCAGCTACGGCGCTATTCCGCTCCATGATAAACCGCTGACGCTCATATTG
CCCTTTGCGACACTCGAACGTGAATTGAGAGCGGCAGGCCAGCTCTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10299.01 Da
• Isoelectric Point: 10.5767

>AT31327 WP_066516583.1 NZ_AP017655:188216-188494 [Sphingobium cloacae]
MPRAVVEDNRRMSLRIRPEQKALLVRAAALKHTDLTTFVTRSALREAENVIEESERTKLSERDSLLVLDLLENPPPANAK
LRAAIAAMPKLA

Nucleotide

• Length: 279 bp

>AT31327 NZ_AP017655:188216-188494 [Sphingobium cloacae]
ATGCCGCGAGCAGTAGTAGAAGACAACAGACGCATGAGCCTGCGGATTCGGCCGGAGCAAAAGGCTTTGCTTGTCCGGGC
GGCTGCGCTCAAGCATACCGATCTCACGACCTTTGTCACACGTTCGGCGCTGCGCGAGGCCGAGAACGTGATCGAAGAGT
CGGAGCGGACGAAACTGAGCGAGAGAGACAGCCTGCTCGTTCTTGATCTGCTCGAGAACCCGCCGCCGGCGAATGCGAAA
CTGCGCGCTGCCATCGCCGCGATGCCGAAGTTGGCATGA

Structures

Toxin

• AlphaFold DB: A0A1E1EY53