Detailed information of TA system TA031326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 94622..95226
• Replicon: chromosome
• Accession: NZ_AP017655
• Organism: Sphingobium cloacae strain JCM 10874

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SCLO_RS00490
• Protein ID: WP_066521555.1
• Coordinates: 94622..94873 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1E1EXX4
• Locus tag: SCLO_RS00495
• Protein ID: WP_066521556.1
• Coordinates: 94885..95226 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCLO_RS00465	90316..91473	+	1158	WP_066521548.1	acyl-CoA dehydrogenase family protein	-
SCLO_RS00470	91523..91846	+	324	WP_083949214.1	SDR family NAD(P)-dependent oxidoreductase	-
SCLO_RS00480	92401..93447	-	1047	WP_066521554.1	IS110 family transposase	-
SCLO_RS00485	94093..94476	-	384	WP_066521569.1	MbcA/ParS/Xre antitoxin family protein	-
SCLO_RS00490	94622..94873	+	252	WP_066521555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SCLO_RS00495	94885..95226	+	342	WP_066521556.1	helix-turn-helix transcriptional regulator	Antitoxin
SCLO_RS00500	95473..96381	+	909	WP_066521558.1	tyrosine-type recombinase/integrase	-
SCLO_RS00505	96386..96946	+	561	Protein_102	transposase	-
SCLO_RS00510	97007..97363	-	357	Protein_103	transposase	-
SCLO_RS00515	97514..97960	+	447	Protein_104	transposase	-
SCLO_RS00520	98972..99406	+	435	WP_048575831.1	PRTRC system protein E	-
SCLO_RS00525	99454..99660	+	207	WP_048575830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	413..209272	208859
	-	inside	IScluster/Tn	-	-	92401..97369	4968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (58-112)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9237.80 Da
• Isoelectric Point: 10.5231

>T31326 WP_066521555.1 NZ_AP017655:94622-94873 [Sphingobium cloacae]
MKPVTYTRAALKALRKMPVDIAERIIRKIEQYAADPTSQANNVTALKGREGIRLRVGDWRVIMNDEGVVLTVLDIGPRGS
IYG

Nucleotide

• Length: 252 bp

>T31326 NZ_AP017655:94622-94873 [Sphingobium cloacae]
ATGAAACCAGTCACCTACACGCGGGCTGCGTTGAAGGCCCTTCGCAAGATGCCCGTGGACATAGCCGAACGGATCATAAG
GAAGATCGAGCAATATGCCGCCGACCCAACCTCGCAGGCCAACAATGTCACGGCTCTTAAGGGGCGGGAAGGTATCCGTT
TAAGAGTGGGTGATTGGCGCGTGATCATGAATGATGAAGGCGTTGTTCTGACGGTTCTCGATATAGGACCACGTGGCAGC
ATCTACGGTTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12424.11 Da
• Isoelectric Point: 4.3005

>AT31326 WP_066521556.1 NZ_AP017655:94885-95226 [Sphingobium cloacae]
MGEMISIPLEEYRSLQAAAEQLADLQAYDRALAQLANGDEEMLPTEFVKRMLSGESPLRAWREFRGLTQTALAEASSVNR
VQIADIESGRKSGSIETVKKLAEALGITLDELI

Nucleotide

• Length: 342 bp

>AT31326 NZ_AP017655:94885-95226 [Sphingobium cloacae]
ATGGGTGAAATGATTTCGATCCCGCTTGAGGAATACCGTAGCTTGCAGGCCGCCGCCGAACAGCTGGCCGATCTGCAAGC
CTATGACCGTGCTCTGGCGCAGTTGGCAAACGGCGATGAGGAAATGCTCCCTACCGAGTTCGTGAAGCGCATGCTCTCCG
GCGAAAGTCCTCTGCGCGCCTGGCGCGAGTTTCGTGGCCTCACCCAGACCGCCCTCGCCGAAGCTTCGAGCGTCAACCGC
GTGCAGATCGCGGATATCGAATCCGGACGGAAGAGCGGTTCAATCGAGACCGTCAAGAAGCTGGCCGAAGCCTTGGGCAT
TACTCTCGATGAACTGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E1EXX4