Detailed information of TA system TA031324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1239293..1239873
• Replicon: chromosome
• Accession: NZ_AP017649
• Organism: Dehalococcoides mccartyi strain UCH-ATV1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DEHALATV1_RS06610
• Protein ID: WP_096476960.1
• Coordinates: 1239293..1239637 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DEHALATV1_RS06615
• Protein ID: WP_096476961.1
• Coordinates: 1239631..1239873 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEHALATV1_RS06575	1234494..1236146	-	1653	WP_096476955.1	recombinase family protein	-
DEHALATV1_RS06580	1236133..1236360	-	228	WP_096476956.1	hypothetical protein	-
DEHALATV1_RS06585	1236378..1236572	-	195	WP_023652786.1	hypothetical protein	-
DEHALATV1_RS06590	1236577..1237200	-	624	WP_096476957.1	ParB N-terminal domain-containing protein	-
DEHALATV1_RS06595	1237187..1237900	-	714	WP_023652788.1	hypothetical protein	-
DEHALATV1_RS07560	1237960..1238127	-	168	WP_155760401.1	hypothetical protein	-
DEHALATV1_RS06600	1238217..1238678	+	462	WP_096476958.1	hypothetical protein	-
DEHALATV1_RS06605	1238762..1239214	+	453	WP_146048216.1	hypothetical protein	-
DEHALATV1_RS06610	1239293..1239637	-	345	WP_096476960.1	endoribonuclease MazF	Toxin
DEHALATV1_RS06615	1239631..1239873	-	243	WP_096476961.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DEHALATV1_RS06620	1240166..1241152	+	987	Protein_1274	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
DEHALATV1_RS06625	1241263..1241673	-	411	Protein_1275	recombinase family protein	-
DEHALATV1_RS06630	1241896..1242390	-	495	WP_096476962.1	hypothetical protein	-
DEHALATV1_RS06640	1243057..1243560	+	504	WP_096476964.1	NUDIX domain-containing protein	-
DEHALATV1_RS06645	1243655..1243933	-	279	Protein_1278	recombinase family protein	-
DEHALATV1_RS06650	1244106..1244576	+	471	WP_096476966.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1190790..1243350	52560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12413.38 Da
• Isoelectric Point: 7.1052

>T31324 WP_096476960.1 NZ_AP017649:c1239637-1239293 [Dehalococcoides mccartyi]
MVNRDYIPQCGDAVRISLNPQAGHEQAGRRPAVVLSPRSYNGKTGLAILCPITRHIKGYPFEVVLPDGLSVSGAILSDQV
KSLDWRVRDAELICTLSDEVISEIMQKLGALLSI

Nucleotide

• Length: 345 bp

>T31324 NZ_AP017649:c1239637-1239293 [Dehalococcoides mccartyi]
ATGGTAAACAGGGATTATATTCCTCAATGCGGGGATGCGGTGCGCATCAGCTTAAATCCGCAGGCAGGCCATGAGCAAGC
CGGGCGCCGTCCCGCCGTCGTGCTCTCCCCGCGAAGCTATAACGGCAAGACAGGGCTTGCTATCCTATGTCCCATTACCA
GGCATATTAAAGGCTATCCCTTCGAGGTAGTGCTTCCTGATGGTTTATCGGTATCTGGAGCAATACTATCCGATCAGGTG
AAGAGCTTAGACTGGCGTGTTCGCGATGCGGAACTGATATGTACCTTATCGGATGAAGTTATCTCGGAGATAATGCAGAA
GCTGGGGGCTCTTTTATCGATATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8716.14 Da
• Isoelectric Point: 8.6020

>AT31324 WP_096476961.1 NZ_AP017649:c1239873-1239631 [Dehalococcoides mccartyi]
MKTLVQKWGNSLALRIPKVFAAEVGLQKETSVEVSLTDGKLIITPVSQPKLTLQQLLAKITKYNLHSEVDTGPAAGDEAW

Nucleotide

• Length: 243 bp

>AT31324 NZ_AP017649:c1239873-1239631 [Dehalococcoides mccartyi]
ATGAAAACCCTTGTTCAGAAATGGGGGAACAGTCTTGCCTTACGTATCCCCAAAGTATTCGCTGCTGAGGTAGGACTTCA
AAAAGAAACATCTGTTGAAGTGTCGTTGACGGACGGCAAGCTTATTATTACTCCGGTATCTCAACCGAAGCTGACACTTC
AACAGCTCCTGGCAAAGATTACCAAATATAATCTGCACAGTGAAGTTGATACCGGCCCTGCTGCGGGGGACGAAGCATGG
TAA