Detailed information of TA system TA031317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 864297..864936
• Replicon: chromosome
• Accession: NZ_AP017624
• Organism: Mycobacterium ulcerans subsp. shinshuense strain ATCC 33728

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: L7VCR2
• Locus tag: SHTP_RS04150
• Protein ID: WP_015357076.1
• Coordinates: 864490..864936 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: SHTP_RS04145
• Protein ID: WP_096369856.1
• Coordinates: 864297..864485 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHTP_RS27900	859810..860065	-	256	Protein_806	transposase	-
SHTP_RS04125	860370..861671	+	1302	WP_157756266.1	hypothetical protein	-
SHTP_RS28135	861722..861892	+	171	WP_157756267.1	pentapeptide repeat-containing protein	-
SHTP_RS26675	861913..862168	+	256	Protein_809	transposase	-
SHTP_RS04135	862168..863155	+	988	Protein_810	ISAs1 family transposase	-
SHTP_RS04140	863332..864195	+	864	WP_096369855.1	pentapeptide repeat-containing protein	-
SHTP_RS04145	864297..864485	+	189	WP_096369856.1	antitoxin	Antitoxin
SHTP_RS04150	864490..864936	+	447	WP_015357076.1	SRPBCC family protein	Toxin
SHTP_RS04155	865048..866301	-	1254	WP_096369857.1	hypothetical protein	-
SHTP_RS04160	866467..867240	+	774	Protein_815	VOC family protein	-
SHTP_RS04165	867311..867745	+	435	WP_012396159.1	hypothetical protein	-
SHTP_RS04170	867751..868224	-	474	WP_012396158.1	VOC family protein	-
SHTP_RS04175	868241..869761	-	1521	WP_096369859.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15861.43 Da
• Isoelectric Point: 8.6574

>T31317 WP_015357076.1 NZ_AP017624:864490-864936 [Mycobacterium ulcerans subsp. shinshuense]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLPETLEKGAVVESIVQVKGMHNRIKWTIVRYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEEGSIVSFDVHLGGPALFGPIGMVVAAALRSDINASLRNFVTVFAPPAAG

Nucleotide

• Length: 447 bp

>T31317 NZ_AP017624:864490-864936 [Mycobacterium ulcerans subsp. shinshuense]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGATCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCAGCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGCCGGCTGCCGGCTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6612.43 Da
• Isoelectric Point: 5.7733

>AT31317 WP_096369856.1 NZ_AP017624:864297-864485 [Mycobacterium ulcerans subsp. shinshuense]
MGILDKVKDLLAQNANKVETVIDKAGEFVDEKTEGKYSDTIHKVQQGAKKVAGPGAAGEQQN

Nucleotide

• Length: 189 bp

>AT31317 NZ_AP017624:864297-864485 [Mycobacterium ulcerans subsp. shinshuense]
ATGGGAATCCTGGACAAGGTGAAAGACCTGCTGGCGCAGAACGCCAACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAAGGGAAGTACTCCGACACCATCCACAAGGTGCAACAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Structures

Toxin

• AlphaFold DB: A0A1B4XZB0