31302

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 74673..75326 Replicon chromosome
Accession NZ_AP017623
Organism Enterococcus faecalis strain W11

Toxin (Protein)


Gene name hicA Uniprot ID R3IHS7
Locus tag EFW11_RS00335 Protein ID WP_002367585.1
Coordinates 75144..75326 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R3IHD2
Locus tag EFW11_RS00330 Protein ID WP_010707936.1
Coordinates 74673..75110 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFW11_RS00315 69951..70649 + 699 WP_096397454.1 N-acetylmannosamine-6-phosphate 2-epimerase -
EFW11_RS00320 70827..73166 + 2340 WP_096397455.1 alpha-glucosidase -
EFW11_RS00325 73710..74627 + 918 WP_096397456.1 helix-turn-helix domain-containing protein -
EFW11_RS00330 74673..75110 - 438 WP_010707936.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EFW11_RS00335 75144..75326 - 183 WP_002367585.1 type II toxin-antitoxin system HicA family toxin Toxin
EFW11_RS00340 75432..76085 - 654 WP_002356081.1 Crp/Fnr family transcriptional regulator -
EFW11_RS00345 76359..77252 + 894 WP_002356084.1 SDR family oxidoreductase -
EFW11_RS00350 77265..77837 + 573 WP_002381343.1 alkaline shock response membrane anchor protein AmaP -
EFW11_RS00355 77850..78041 + 192 WP_002356087.1 DUF2273 domain-containing protein -
EFW11_RS00360 78054..78566 + 513 WP_002383483.1 Asp23/Gls24 family envelope stress response protein -
EFW11_RS00365 78625..79185 + 561 WP_002383482.1 Asp23/Gls24 family envelope stress response protein -
EFW11_RS00370 79209..79451 + 243 WP_002356092.1 GlsB/YeaQ/YmgE family stress response membrane protein -
EFW11_RS00375 79616..80131 + 516 WP_096397457.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(11-71)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6633.06 Da        Isoelectric Point: 10.8756

>T31302 WP_002367585.1 NZ_AP017623:c75326-75144 [Enterococcus faecalis]
MPMTQKQMVKLLKKHGWLPTDGGKGSHIKMEMSGKRPIIIPHGELKKGTEIGILKEAGLK

Download         Length: 183 bp

>T31302 NZ_AP017623:c75326-75144 [Enterococcus faecalis]
ATGCCAATGACACAAAAACAAATGGTCAAATTGCTAAAAAAGCACGGTTGGTTACCAACCGATGGTGGAAAAGGCTCCCA
TATAAAAATGGAGATGTCTGGCAAAAGACCCATCATTATCCCACACGGAGAATTGAAAAAAGGAACCGAAATAGGGATTT
TAAAAGAGGCAGGGCTGAAATAG

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16267.27 Da        Isoelectric Point: 4.2758

>AT31302 WP_010707936.1 NZ_AP017623:c75110-74673 [Enterococcus faecalis]
MLISYPACFYSVENGYYVYFPDIGGSGTQGDTIPDAIAMASDYLGMMLSDDIEHQRTIHQPSPINTLSLEDNNPFRDDDD
FNFNLADSFVSMVNVELNDYLGTNTLVKKTVTIPKWSDELGKKNKLNFSKTLTDAIVEKSLHVLK

Download         Length: 438 bp

>AT31302 NZ_AP017623:c75110-74673 [Enterococcus faecalis]
ATGCTAATTAGTTATCCTGCATGTTTCTACAGTGTCGAGAATGGTTACTATGTTTATTTCCCAGATATAGGTGGTTCTGG
TACGCAAGGAGATACAATTCCTGATGCAATTGCGATGGCTTCCGATTATTTAGGTATGATGTTATCTGATGATATTGAAC
ATCAAAGAACAATCCACCAGCCATCTCCTATCAATACGTTGTCATTAGAAGATAATAATCCCTTTAGAGATGACGATGAC
TTCAATTTTAACCTTGCAGACTCGTTTGTTTCTATGGTAAATGTAGAACTAAATGATTATTTAGGAACAAATACGTTAGT
CAAAAAAACCGTCACCATTCCCAAGTGGTCTGATGAACTCGGGAAAAAAAATAAATTGAATTTTTCTAAAACCTTGACCG
ATGCCATCGTTGAAAAGTCGCTACATGTTTTAAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3IHS7

Antitoxin

Source ID Structure
AlphaFold DB R3IHD2

References