Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4984077..4984653 Replicon chromosome
Accession NZ_AP017620
Organism Escherichia coli strain MRY15-131

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag CPY99_RS25185 Protein ID WP_001131965.1
Coordinates 4984077..4984364 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag CPY99_RS25190 Protein ID WP_000063158.1
Coordinates 4984351..4984653 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPY99_RS25160 4979486..4980898 - 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -
CPY99_RS25165 4981075..4981239 + 165 WP_000394263.1 DUF1127 domain-containing protein -
CPY99_RS25170 4981281..4981619 - 339 WP_000132633.1 endoribonuclease SymE -
CPY99_RS25175 4981834..4982502 - 669 Protein_4635 DUF3387 domain-containing protein -
CPY99_RS27120 4982496..4982669 - 174 Protein_4636 SAM-dependent DNA methyltransferase -
CPY99_RS25180 4982714..4983880 - 1167 WP_000800823.1 restriction endonuclease -
CPY99_RS25185 4984077..4984364 + 288 WP_001131965.1 BrnT family toxin Toxin
CPY99_RS25190 4984351..4984653 + 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
CPY99_RS25195 4984687..4985643 - 957 WP_001361361.1 GTPase -
CPY99_RS25200 4985654..4985857 - 204 WP_000467859.1 YbdD/YjiX family protein -
CPY99_RS25205 4985907..4988057 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
CPY99_RS25215 4988325..4989545 - 1221 WP_000873756.1 amino acid deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T31294 WP_001131965.1 NZ_AP017620:4984077-4984364 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T31294 NZ_AP017620:4984077-4984364 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT31294 WP_000063158.1 NZ_AP017620:4984351-4984653 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT31294 NZ_AP017620:4984351-4984653 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References