Detailed information of TA system TA031288

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4101403..4102201
• Replicon: chromosome
• Accession: NZ_AP017620
• Organism: Escherichia coli strain MRY15-131

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CPY99_RS20730
• Protein ID: WP_061092894.1
• Coordinates: 4101824..4102201 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CPY99_RS20725
• Protein ID: WP_061092893.1
• Coordinates: 4101403..4101777 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPY99_RS20690	4097068..4097748	+	681	WP_001282921.1	WYL domain-containing protein	-
CPY99_RS20695	4097896..4098573	+	678	WP_001097302.1	hypothetical protein	-
CPY99_RS20700	4098579..4098812	+	234	WP_001278293.1	DUF905 family protein	-
CPY99_RS20705	4098902..4099720	+	819	WP_001175165.1	DUF945 domain-containing protein	-
CPY99_RS20710	4099986..4100465	+	480	WP_000706978.1	antirestriction protein	-
CPY99_RS20715	4100480..4100956	+	477	WP_001186188.1	RadC family protein	-
CPY99_RS20720	4101019..4101240	+	222	WP_061092892.1	DUF987 domain-containing protein	-
CPY99_RS20725	4101403..4101777	+	375	WP_061092893.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CPY99_RS20730	4101824..4102201	+	378	WP_061092894.1	TA system toxin CbtA family protein	Toxin
CPY99_RS20735	4102198..4102686	+	489	WP_061092895.1	hypothetical protein	-
CPY99_RS20740	4102698..4102895	+	198	WP_012908799.1	DUF957 domain-containing protein	-
CPY99_RS20745	4102980..4103822	+	843	WP_061092922.1	DUF4942 domain-containing protein	-
CPY99_RS20755	4104090..4104236	+	147	WP_001328030.1	hypothetical protein	-
CPY99_RS20760	4104620..4105804	+	1185	WP_001172864.1	sugar efflux transporter	-
CPY99_RS20765	4105915..4106838	+	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4053192..4115447	62255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.15 Da
• Isoelectric Point: 7.8045

>T31288 WP_061092894.1 NZ_AP017620:4101824-4102201 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T31288 NZ_AP017620:4101824-4102201 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13752.53 Da
• Isoelectric Point: 6.5924

>AT31288 WP_061092893.1 NZ_AP017620:4101403-4101777 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNHLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT31288 NZ_AP017620:4101403-4101777 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCACCTGCATTACCTTGCCGACCGCGCGGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA