Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3819842..3820473 Replicon chromosome
Accession NZ_AP017620
Organism Escherichia coli strain MRY15-131

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag CPY99_RS19335 Protein ID WP_001260301.1
Coordinates 3820198..3820473 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CPY99_RS19330 Protein ID WP_000593555.1
Coordinates 3819842..3820201 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPY99_RS19305 3815975..3816919 + 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
CPY99_RS19310 3816916..3817749 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CPY99_RS19315 3817749..3818513 + 765 WP_001136211.1 nickel import ATP-binding protein NikD -
CPY99_RS19320 3818510..3819316 + 807 WP_061092823.1 nickel import ATP-binding protein NikE -
CPY99_RS19325 3819321..3819722 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CPY99_RS19330 3819842..3820201 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CPY99_RS19335 3820198..3820473 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
CPY99_RS19340 3820546..3821670 - 1125 WP_001314210.1 ABC transporter permease -
CPY99_RS19345 3821670..3824405 - 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
CPY99_RS19350 3824402..3825469 - 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T31286 WP_001260301.1 NZ_AP017620:c3820473-3820198 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T31286 NZ_AP017620:c3820473-3820198 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT31286 WP_000593555.1 NZ_AP017620:c3820201-3819842 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT31286 NZ_AP017620:c3820201-3819842 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References