Detailed information of TA system TA031283

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3284359..3285191
• Replicon: chromosome
• Accession: NZ_AP017620
• Organism: Escherichia coli strain MRY15-131

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: CPY99_RS16490
• Protein ID: WP_000854753.1
• Coordinates: 3284817..3285191 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: CPY99_RS16485
• Protein ID: WP_001540478.1
• Coordinates: 3284359..3284727 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPY99_RS16450	3280191..3280871	+	681	WP_001282919.1	WYL domain-containing protein	-
CPY99_RS16455	3281019..3281696	+	678	WP_001097301.1	hypothetical protein	-
CPY99_RS16460	3281702..3281935	+	234	WP_001278283.1	DUF905 family protein	-
CPY99_RS16465	3282025..3282843	+	819	WP_001234382.1	DUF945 domain-containing protein	-
CPY99_RS16470	3282935..3283420	+	486	WP_023909076.1	antirestriction protein	-
CPY99_RS16475	3283436..3283912	+	477	WP_061092719.1	RadC family protein	-
CPY99_RS16480	3283975..3284196	+	222	WP_000692311.1	DUF987 domain-containing protein	-
CPY99_RS16485	3284359..3284727	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CPY99_RS16490	3284817..3285191	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
CPY99_RS16495	3285188..3285676	+	489	WP_000777545.1	hypothetical protein	-
CPY99_RS16500	3285688..3285885	+	198	WP_000839281.1	DUF957 domain-containing protein	-
CPY99_RS16505	3285982..3286551	+	570	WP_001290252.1	DUF4942 domain-containing protein	-
CPY99_RS16510	3286894..3287064	+	171	Protein_3068	IS110 family transposase	-
CPY99_RS16515	3287875..3288858	+	984	WP_001355482.1	KpsF/GutQ family sugar-phosphate isomerase	-
CPY99_RS16520	3288930..3290078	+	1149	WP_000905925.1	capsule polysaccharide transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T31283 WP_000854753.1 NZ_AP017620:3284817-3285191 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T31283 NZ_AP017620:3284817-3285191 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT31283 WP_001540478.1 NZ_AP017620:3284359-3284727 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT31283 NZ_AP017620:3284359-3284727 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68