31282

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 3250534..3251869 Replicon chromosome
Accession NZ_AP017620
Organism Escherichia coli strain MRY15-131

Toxin (Protein)


Gene name sanaT Uniprot ID A0A0K4CDG2
Locus tag CPY99_RS16305 Protein ID WP_001440173.1
Coordinates 3250922..3251869 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A0K6A7X2
Locus tag CPY99_RS16300 Protein ID WP_000208386.1
Coordinates 3250534..3250929 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPY99_RS16250 3246020..3246505 + 486 WP_000213705.1 antirestriction protein -
CPY99_RS16260 3246521..3246997 + 477 WP_001186715.1 RadC family protein -
CPY99_RS16265 3247066..3247287 + 222 WP_000692345.1 DUF987 domain-containing protein -
CPY99_RS16270 3247306..3247950 + 645 WP_000086770.1 hypothetical protein -
CPY99_RS16275 3248000..3248368 + 369 WP_001285113.1 type IV toxin-antitoxin system YeeU family antitoxin -
CPY99_RS16280 3248458..3248835 + 378 WP_000854719.1 TA system toxin CbtA family protein -
CPY99_RS16285 3248832..3249320 + 489 WP_000761679.1 hypothetical protein -
CPY99_RS16290 3249338..3249535 + 198 WP_000839266.1 DUF957 domain-containing protein -
CPY99_RS16295 3249620..3250465 + 846 WP_001440175.1 DUF4942 domain-containing protein -
CPY99_RS16300 3250534..3250929 + 396 WP_000208386.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
CPY99_RS16305 3250922..3251869 + 948 WP_001440173.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
CPY99_RS16310 3252273..3253538 + 1266 WP_096427322.1 integrase arm-type DNA-binding domain-containing protein -
CPY99_RS16315 3253860..3255359 - 1500 WP_096427323.1 phosphoethanolamine transferase -
CPY99_RS16320 3255541..3256281 - 741 WP_001045031.1 porin family protein -
CPY99_RS16325 3256391..3256867 - 477 WP_001275824.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - sfaX 3242030..3279003 36973


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(36-302)

Antitoxin

Protein of unknown function DUF6088

(10-129)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 36284.66 Da        Isoelectric Point: 6.2532

>T31282 WP_001440173.1 NZ_AP017620:3250922-3251869 [Escherichia coli]
MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRNRKRKIRKEIVQIITDVLHNSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
DIYSLVTELTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQHPQFRNSPVDELKKGLDALRDDPVHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN

Download         Length: 948 bp

>T31282 NZ_AP017620:3250922-3251869 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCAACGCACTGGATATTGAATCTGTCGCCATCGTCGAAAAAGATTA
TTTTGTGGTTAATCTTTTAAAGTTACTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TGTCAAAAGCTGGTATCTCATTGAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAAACAGGAAAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTCTTCATAATTCTGACATTTT
CAGCCTTGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAGATCCCCGTACGTTATCCCCAGACAT
TTGCCCAGGCCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAACCACTCTGCTGGAACACCCGGAAAGCAGA
GATATTTACTCACTCGTCACGGAGTTAACGGGTAATGGCACACCTGTAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATATCCATGATGCGAAGAACAGCCATACTTCTTCGCAATCCCGAACAACAGGATGATGAGTCCC
TGGTACGCCACATTTACGACAATTATTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCAGTACTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGACATTGAGCGCTATGGTAACCAGCACCCACAATTTCGTAACTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATGATCCTGTACACAAATTGCGTTTTCAACGATTTGTTAAACCGATGGTATTTGGTAATA
CACAGATTGACTGGGAAGAAGCCTACTCCTGTTTCAGACAAACAGCGTTGAGTGTTCTGACCAACTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14639.00 Da        Isoelectric Point: 11.0607

>AT31282 WP_000208386.1 NZ_AP017620:3250534-3250929 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITE
ALDRLNIRYSLIGATECYNKGQSTQIPVSPEFKTPPRFKRVLVVGKSKINA

Download         Length: 396 bp

>AT31282 NZ_AP017620:3250534-3250929 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAGCGTTATGTGTTCACCCGGGATGACTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACGCTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGCAACGTTATTACAGGAAAAATCATGCCTGCAGCACCTGGTGGCTCTGCCGCTGTAATCACCGAA
GCCCTGGATCGTTTGAATATCAGATACAGCTTGATCGGCGCAACAGAATGCTACAATAAAGGTCAGTCCACACAGATTCC
GGTATCTCCGGAGTTCAAAACACCTCCTCGCTTTAAAAGAGTGTTAGTTGTAGGGAAAAGTAAAATCAATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CDG2

Antitoxin

Source ID Structure
AlphaFold DB A0A0K6A7X2

References