31278

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2077676..2078460 Replicon chromosome
Accession NZ_AP017620
Organism Escherichia coli strain MRY15-131

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag CPY99_RS10630 Protein ID WP_000613626.1
Coordinates 2077966..2078460 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag CPY99_RS10625 Protein ID WP_001110447.1
Coordinates 2077676..2077969 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPY99_RS10610 2072826..2073785 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
CPY99_RS10620 2074358..2077543 + 3186 WP_061092653.1 ribonuclease E -
CPY99_RS10625 2077676..2077969 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
CPY99_RS10630 2077966..2078460 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
CPY99_RS10635 2078555..2079508 - 954 WP_001212762.1 flagellar hook-associated protein FlgL -
CPY99_RS10640 2079520..2081163 - 1644 WP_061092652.1 flagellar hook-associated protein FlgK -
CPY99_RS10645 2081229..2082170 - 942 WP_001305885.1 flagellar assembly peptidoglycan hydrolase FlgJ -
CPY99_RS10650 2082170..2083267 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(81-144)

Antitoxin

(14-88)


Sequences


Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T31278 WP_000613626.1 NZ_AP017620:2077966-2078460 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T31278 NZ_AP017620:2077966-2078460 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT31278 WP_001110447.1 NZ_AP017620:2077676-2077969 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT31278 NZ_AP017620:2077676-2077969 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0T0H9


Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References