31256

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5058691..5059267 Replicon chromosome
Accession NZ_AP017617
Organism Escherichia coli strain MRY15-117

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag CPZ03_RS25565 Protein ID WP_001131965.1
Coordinates 5058691..5058978 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag CPZ03_RS25570 Protein ID WP_000063158.1
Coordinates 5058965..5059267 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ03_RS25540 5054100..5055512 - 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -
CPZ03_RS25545 5055689..5055853 + 165 WP_000394263.1 DUF1127 domain-containing protein -
CPZ03_RS25550 5055895..5056233 - 339 WP_000132633.1 endoribonuclease SymE -
CPZ03_RS25555 5056448..5057116 - 669 Protein_4707 DUF3387 domain-containing protein -
CPZ03_RS27515 5057110..5057283 - 174 Protein_4708 SAM-dependent DNA methyltransferase -
CPZ03_RS25560 5057328..5058494 - 1167 WP_000800823.1 restriction endonuclease -
CPZ03_RS25565 5058691..5058978 + 288 WP_001131965.1 BrnT family toxin Toxin
CPZ03_RS25570 5058965..5059267 + 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
CPZ03_RS25575 5059301..5060257 - 957 WP_001361361.1 GTPase -
CPZ03_RS25580 5060268..5060471 - 204 WP_000467859.1 YbdD/YjiX family protein -
CPZ03_RS25585 5060521..5062671 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
CPZ03_RS25595 5062939..5064159 - 1221 WP_000873756.1 amino acid deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T31256 WP_001131965.1 NZ_AP017617:5058691-5058978 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T31256 NZ_AP017617:5058691-5058978 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT31256 WP_000063158.1 NZ_AP017617:5058965-5059267 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT31256 NZ_AP017617:5058965-5059267 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References