31251

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4464362..4464978 Replicon chromosome
Accession NZ_AP017617
Organism Escherichia coli strain MRY15-117

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag CPZ03_RS22520 Protein ID WP_001129490.1
Coordinates 4464604..4464978 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag CPZ03_RS22515 Protein ID WP_001275523.1
Coordinates 4464362..4464604 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ03_RS22485 4459597..4460034 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
CPZ03_RS22490 4460079..4461020 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
CPZ03_RS22495 4461436..4463250 + 1815 WP_000226324.1 hypothetical protein -
CPZ03_RS22510 4463924..4464142 + 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
CPZ03_RS22515 4464362..4464604 + 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
CPZ03_RS22520 4464604..4464978 + 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
CPZ03_RS22525 4465015..4465944 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
CPZ03_RS22530 4465941..4466576 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
CPZ03_RS22535 4466573..4467475 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T31251 WP_001129490.1 NZ_AP017617:4464604-4464978 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T31251 NZ_AP017617:4464604-4464978 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT31251 WP_001275523.1 NZ_AP017617:4464362-4464604 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT31251 NZ_AP017617:4464362-4464604 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References