Detailed information of TA system TA031250

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4172519..4173317
• Replicon: chromosome
• Accession: NZ_AP017617
• Organism: Escherichia coli strain MRY15-117

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CPZ03_RS21095
• Protein ID: WP_061092894.1
• Coordinates: 4172940..4173317 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CPZ03_RS21090
• Protein ID: WP_061092893.1
• Coordinates: 4172519..4172893 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ03_RS21055	4168184..4168864	+	681	WP_001282921.1	WYL domain-containing protein	-
CPZ03_RS21060	4169012..4169689	+	678	WP_001097302.1	hypothetical protein	-
CPZ03_RS21065	4169695..4169928	+	234	WP_001278293.1	DUF905 family protein	-
CPZ03_RS21070	4170018..4170836	+	819	WP_001175165.1	DUF945 domain-containing protein	-
CPZ03_RS21075	4171102..4171581	+	480	WP_000706978.1	antirestriction protein	-
CPZ03_RS21080	4171596..4172072	+	477	WP_001186188.1	RadC family protein	-
CPZ03_RS21085	4172135..4172356	+	222	WP_061092892.1	DUF987 domain-containing protein	-
CPZ03_RS21090	4172519..4172893	+	375	WP_061092893.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CPZ03_RS21095	4172940..4173317	+	378	WP_061092894.1	TA system toxin CbtA family protein	Toxin
CPZ03_RS21100	4173314..4173802	+	489	WP_061092895.1	hypothetical protein	-
CPZ03_RS21105	4173814..4174011	+	198	WP_012908799.1	DUF957 domain-containing protein	-
CPZ03_RS21110	4174096..4174938	+	843	WP_061092922.1	DUF4942 domain-containing protein	-
CPZ03_RS21120	4175206..4175352	+	147	WP_001328030.1	hypothetical protein	-
CPZ03_RS21125	4175736..4176920	+	1185	WP_001172864.1	sugar efflux transporter	-
CPZ03_RS21130	4177031..4177954	+	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.15 Da
• Isoelectric Point: 7.8045

>T31250 WP_061092894.1 NZ_AP017617:4172940-4173317 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T31250 NZ_AP017617:4172940-4173317 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13752.53 Da
• Isoelectric Point: 6.5924

>AT31250 WP_061092893.1 NZ_AP017617:4172519-4172893 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNHLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT31250 NZ_AP017617:4172519-4172893 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCACCTGCATTACCTTGCCGACCGCGCGGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA