31248

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3890956..3891587 Replicon chromosome
Accession NZ_AP017617
Organism Escherichia coli strain MRY15-117

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag CPZ03_RS19705 Protein ID WP_001260301.1
Coordinates 3891312..3891587 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CPZ03_RS19700 Protein ID WP_000593555.1
Coordinates 3890956..3891315 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ03_RS19675 3887089..3888033 + 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
CPZ03_RS19680 3888030..3888863 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CPZ03_RS19685 3888863..3889627 + 765 WP_001136211.1 nickel import ATP-binding protein NikD -
CPZ03_RS19690 3889624..3890430 + 807 WP_061092823.1 nickel import ATP-binding protein NikE -
CPZ03_RS19695 3890435..3890836 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CPZ03_RS19700 3890956..3891315 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CPZ03_RS19705 3891312..3891587 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
CPZ03_RS19710 3891660..3892784 - 1125 WP_001314210.1 ABC transporter permease -
CPZ03_RS19715 3892784..3895519 - 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
CPZ03_RS19720 3895516..3896583 - 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T31248 WP_001260301.1 NZ_AP017617:c3891587-3891312 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T31248 NZ_AP017617:c3891587-3891312 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT31248 WP_000593555.1 NZ_AP017617:c3891315-3890956 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT31248 NZ_AP017617:c3891315-3890956 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References