Detailed information of TA system TA031244

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3297683..3298515
• Replicon: chromosome
• Accession: NZ_AP017617
• Organism: Escherichia coli strain MRY15-117

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X9SFA4
• Locus tag: CPZ03_RS16550
• Protein ID: WP_001094442.1
• Coordinates: 3298141..3298515 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CPZ03_RS16545
• Protein ID: WP_096485666.1
• Coordinates: 3297683..3298051 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ03_RS16510	3293574..3294254	+	681	WP_001282919.1	WYL domain-containing protein	-
CPZ03_RS16515	3294402..3295079	+	678	WP_001097305.1	hypothetical protein	-
CPZ03_RS16520	3295085..3295318	+	234	WP_001278287.1	DUF905 family protein	-
CPZ03_RS16525	3295408..3296226	+	819	WP_001175169.1	DUF945 domain-containing protein	-
CPZ03_RS16530	3296318..3296803	+	486	WP_000206658.1	antirestriction protein	-
CPZ03_RS16535	3296819..3297295	+	477	WP_001367707.1	RadC family protein	-
CPZ03_RS16540	3297382..3297603	+	222	WP_000691819.1	DUF987 domain-containing protein	-
CPZ03_RS16545	3297683..3298051	+	369	WP_096485666.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CPZ03_RS16550	3298141..3298515	+	375	WP_001094442.1	TA system toxin CbtA family protein	Toxin
CPZ03_RS16555	3298512..3299000	+	489	WP_000777553.1	hypothetical protein	-
CPZ03_RS16560	3299012..3299209	+	198	WP_000839281.1	DUF957 domain-containing protein	-
CPZ03_RS16565	3299306..3299875	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
CPZ03_RS16570	3300723..3301310	+	588	WP_031250069.1	hypothetical protein	-
CPZ03_RS16575	3301656..3302840	+	1185	WP_023892874.1	site-specific integrase	-
CPZ03_RS16580	3302895..3303251	-	357	WP_032165636.1	Arm DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14072.01 Da
• Isoelectric Point: 7.8281

>T31244 WP_001094442.1 NZ_AP017617:3298141-3298515 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T31244 NZ_AP017617:3298141-3298515 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13675.50 Da
• Isoelectric Point: 6.4768

>AT31244 WP_096485666.1 NZ_AP017617:3297683-3298051 [Escherichia coli]
VSDTLPGTTHPDDNNDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDLAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT31244 NZ_AP017617:3297683-3298051 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCACCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCTGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2X9SFA4