Detailed information of TA system TA031242

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3249813..3250648
• Replicon: chromosome
• Accession: NZ_AP017617
• Organism: Escherichia coli strain MRY15-117

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1V1GYU4
• Locus tag: CPZ03_RS16240
• Protein ID: WP_000854719.1
• Coordinates: 3250271..3250648 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CPZ03_RS16235
• Protein ID: WP_001285113.1
• Coordinates: 3249813..3250181 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ03_RS16190	3245104..3245784	+	681	WP_001282919.1	WYL domain-containing protein	-
CPZ03_RS16195	3245932..3246609	+	678	WP_001097304.1	hypothetical protein	-
CPZ03_RS16200	3246615..3246848	+	234	WP_001278282.1	DUF905 family protein	-
CPZ03_RS16205	3246938..3247756	+	819	WP_096485662.1	DUF945 domain-containing protein	-
CPZ03_RS16210	3247833..3248318	+	486	WP_000213705.1	antirestriction protein	-
CPZ03_RS16220	3248334..3248810	+	477	WP_001186715.1	RadC family protein	-
CPZ03_RS16225	3248879..3249100	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CPZ03_RS16230	3249119..3249763	+	645	WP_000086770.1	hypothetical protein	-
CPZ03_RS16235	3249813..3250181	+	369	WP_001285113.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CPZ03_RS16240	3250271..3250648	+	378	WP_000854719.1	TA system toxin CbtA family protein	Toxin
CPZ03_RS16245	3250645..3251133	+	489	WP_000761679.1	hypothetical protein	-
CPZ03_RS16250	3251151..3251348	+	198	WP_000839266.1	DUF957 domain-containing protein	-
CPZ03_RS16255	3251433..3252278	+	846	WP_001440175.1	DUF4942 domain-containing protein	-
CPZ03_RS16260	3252347..3252742	+	396	WP_000208386.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
CPZ03_RS16265	3252735..3253682	+	948	WP_001440173.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CPZ03_RS16270	3254086..3255351	+	1266	WP_096427322.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14059.10 Da
• Isoelectric Point: 8.2904

>T31242 WP_000854719.1 NZ_AP017617:3250271-3250648 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T31242 NZ_AP017617:3250271-3250648 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13693.33 Da
• Isoelectric Point: 5.4970

>AT31242 WP_001285113.1 NZ_AP017617:3249813-3250181 [Escherichia coli]
VSDSLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYARGLTCEADTLGSCGYVYLAVYPTSETKT

Nucleotide

• Length: 369 bp

>AT31242 NZ_AP017617:3249813-3250181 [Escherichia coli]
GTGTCAGACTCACTCCATGAAACAAATTATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A1V1GYU4