Detailed information of TA system TA031229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 898806..899511
• Replicon: chromosome
• Accession: NZ_AP017617
• Organism: Escherichia coli strain MRY15-117

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: CPZ03_RS04630
• Protein ID: WP_000539524.1
• Coordinates: 899125..899511 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CPZ03_RS04625
• Protein ID: WP_001280945.1
• Coordinates: 898806..899135 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ03_RS04610	893975..894886	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
CPZ03_RS04615	895063..897411	+	2349	WP_000950331.1	EAL domain-containing protein	-
CPZ03_RS04620	897419..898736	+	1318	Protein_848	diguanylate cyclase	-
CPZ03_RS04625	898806..899135	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CPZ03_RS04630	899125..899511	-	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPZ03_RS04635	899737..901062	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CPZ03_RS04640	901275..901658	+	384	WP_000497137.1	biofilm formation regulator BssR	-
CPZ03_RS04645	901769..902884	+	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
CPZ03_RS04650	902881..903507	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T31229 WP_000539524.1 NZ_AP017617:c899511-899125 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T31229 NZ_AP017617:c899511-899125 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT31229 WP_001280945.1 NZ_AP017617:c899135-898806 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT31229 NZ_AP017617:c899135-898806 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7