Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 472472..473090 Replicon chromosome
Accession NZ_AP017617
Organism Escherichia coli strain MRY15-117

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CPZ03_RS02345 Protein ID WP_001291435.1
Coordinates 472472..472690 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CPZ03_RS02350 Protein ID WP_000344800.1
Coordinates 472716..473090 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ03_RS02310 467761..468333 + 573 WP_000779831.1 YbaY family lipoprotein -
CPZ03_RS02315 468364..468675 - 312 WP_000409911.1 MGMT family protein -
CPZ03_RS02325 469054..469407 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CPZ03_RS02330 469449..470999 - 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CPZ03_RS02335 471163..471633 - 471 WP_000136192.1 YlaC family protein -
CPZ03_RS02340 471749..472300 - 552 WP_000102574.1 maltose O-acetyltransferase -
CPZ03_RS02345 472472..472690 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CPZ03_RS02350 472716..473090 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CPZ03_RS02355 473636..476785 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CPZ03_RS02360 476808..478001 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T31227 WP_001291435.1 NZ_AP017617:c472690-472472 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T31227 NZ_AP017617:c472690-472472 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT31227 WP_000344800.1 NZ_AP017617:c473090-472716 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT31227 NZ_AP017617:c473090-472716 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References