Detailed information of TA system TA031226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 438163..439000
• Replicon: chromosome
• Accession: NZ_AP017617
• Organism: Escherichia coli strain MRY15-117

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A1M0D5U3
• Locus tag: CPZ03_RS02165
• Protein ID: WP_000227782.1
• Coordinates: 438163..438705 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: CPZ03_RS02170
• Protein ID: WP_001353405.1
• Coordinates: 438689..439000 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ03_RS02140	433241..433831	-	591	WP_001276320.1	protein deglycase YajL	-
CPZ03_RS02145	433794..434705	-	912	WP_000705841.1	2-dehydropantoate 2-reductase	-
CPZ03_RS02150	434873..435364	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
CPZ03_RS02155	435492..436856	-	1365	WP_001000978.1	MFS transporter	-
CPZ03_RS02160	437263..438107	+	845	Protein_404	sel1 repeat family protein	-
CPZ03_RS02165	438163..438705	-	543	WP_000227782.1	GNAT family N-acetyltransferase	Toxin
CPZ03_RS02170	438689..439000	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
CPZ03_RS02175	439185..440075	-	891	WP_000971327.1	heme o synthase	-
CPZ03_RS02180	440087..440416	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CPZ03_RS02185	440416..441030	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CPZ03_RS02190	441020..443011	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CPZ03_RS02195	443033..443980	-	948	WP_001239437.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19957.31 Da
• Isoelectric Point: 9.1763

>T31226 WP_000227782.1 NZ_AP017617:c438705-438163 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T31226 NZ_AP017617:c438705-438163 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT31226 WP_001353405.1 NZ_AP017617:c439000-438689 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT31226 NZ_AP017617:c439000-438689 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A1M0D5U3

Antitoxin

• AlphaFold DB: A0A829G7G3