Detailed information of TA system TA031225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 53545..54067
• Replicon: plasmid pMRY16-002_4
• Accession: NZ_AP017614
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CPZ02_RS28175
• Protein ID: WP_096488323.1
• Coordinates: 53545..53826 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: CPZ02_RS28180
• Protein ID: WP_000121741.1
• Coordinates: 53816..54067 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS28140	49694..50014	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
CPZ02_RS28145	50085..50273	-	189	WP_050567133.1	hypothetical protein	-
CPZ02_RS28150	50375..50959	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
CPZ02_RS28155	50980..51378	-	399	WP_001153665.1	hypothetical protein	-
CPZ02_RS28160	51497..51934	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
CPZ02_RS28165	51940..53175	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
CPZ02_RS28170	53178..53477	-	300	WP_000835764.1	IncN plasmid KikA protein	-
CPZ02_RS28175	53545..53826	-	282	WP_096488323.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPZ02_RS28180	53816..54067	-	252	WP_000121741.1	hypothetical protein	Antitoxin
CPZ02_RS28185	54167..54802	-	636	WP_015059536.1	hypothetical protein	-
CPZ02_RS28190	54875..55162	-	288	WP_001032611.1	EexN family lipoprotein	-
CPZ02_RS28195	55175..55429	-	255	WP_001043555.1	EexN family lipoprotein	-
CPZ02_RS28200	55431..56072	-	642	WP_001425343.1	conjugal transfer protein TrbJ	-
CPZ02_RS28205	56078..57073	-	996	WP_001028543.1	type IV secretion system protein	-
CPZ02_RS28210	57077..57334	-	258	WP_000739144.1	hypothetical protein	-
CPZ02_RS28215	57331..57633	-	303	WP_001360345.1	hypothetical protein	-
CPZ02_RS28220	57614..57871	-	258	WP_001542015.1	hypothetical protein	-
CPZ02_RS28225	57904..58350	-	447	WP_001243165.1	hypothetical protein	-
CPZ02_RS29125	58361..58531	-	171	WP_000550720.1	hypothetical protein	-
CPZ02_RS28230	58535..58978	-	444	WP_000964330.1	NfeD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61805	61805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10959.82 Da
• Isoelectric Point: 10.4997

>T31225 WP_096488323.1 NZ_AP017614:c53826-53545 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRIPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T31225 NZ_AP017614:c53826-53545 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCATCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT31225 WP_000121741.1 NZ_AP017614:c54067-53816 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT31225 NZ_AP017614:c54067-53816 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Antitoxin

• AlphaFold DB: U9Y7J1