Detailed information of TA system TA031224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 7527..8028
• Replicon: plasmid pMRY16-002_4
• Accession: NZ_AP017614
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: CPZ02_RS27900
• Protein ID: WP_000702247.1
• Coordinates: 7527..7787 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: CPZ02_RS27905
• Protein ID: WP_001132407.1
• Coordinates: 7777..8028 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS27850	3123..3284	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
CPZ02_RS27855	3434..4030	+	597	WP_000125165.1	hypothetical protein	-
CPZ02_RS29190	4048..4332	-	285	WP_001162371.1	hypothetical protein	-
CPZ02_RS27865	4406..5071	-	666	WP_000532148.1	P-loop NTPase	-
CPZ02_RS27875	5669..5998	+	330	WP_000855120.1	hypothetical protein	-
CPZ02_RS27885	6580..6903	+	324	WP_000427403.1	hypothetical protein	-
CPZ02_RS27890	6945..7154	+	210	WP_000866036.1	hypothetical protein	-
CPZ02_RS27895	7251..7475	+	225	WP_000814381.1	hypothetical protein	-
CPZ02_RS27900	7527..7787	+	261	WP_000702247.1	hypothetical protein	Toxin
CPZ02_RS27905	7777..8028	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
CPZ02_RS27920	8578..8856	+	279	WP_000545938.1	hypothetical protein	-
CPZ02_RS27925	9276..9542	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
CPZ02_RS27930	9649..9831	-	183	WP_001481829.1	hypothetical protein	-
CPZ02_RS27935	10389..10571	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
CPZ02_RS27940	10596..11033	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
CPZ02_RS27945	11164..11490	-	327	WP_063078684.1	hypothetical protein	-
CPZ02_RS27950	11510..12217	-	708	WP_021503228.1	hypothetical protein	-
CPZ02_RS27955	12219..12971	-	753	WP_000797966.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..61805	61805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T31224 WP_000702247.1 NZ_AP017614:7527-7787 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T31224 NZ_AP017614:7527-7787 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT31224 WP_001132407.1 NZ_AP017614:7777-8028 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT31224 NZ_AP017614:7777-8028 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0