Detailed information of TA system TA031218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 85255..85814
• Replicon: plasmid pMRY16-002_1
• Accession: NZ_AP017611
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CPZ02_RS25895
• Protein ID: WP_001620845.1
• Coordinates: 85255..85530 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: CPZ02_RS25900
• Protein ID: WP_001178089.1
• Coordinates: 85530..85814 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS25875	83133..83498	+	366	WP_000124086.1	hypothetical protein	-
CPZ02_RS25880	83498..84675	+	1178	Protein_69	IS91 family transposase	-
CPZ02_RS25890	84868..85149	-	282	WP_021533574.1	hypothetical protein	-
CPZ02_RS25895	85255..85530	-	276	WP_001620845.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPZ02_RS25900	85530..85814	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
CPZ02_RS25905	86082..86441	+	360	Protein_73	recombinase family protein	-
CPZ02_RS25910	86495..87199	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
CPZ02_RS25915	87266..88123	-	858	WP_122997250.1	incFII family plasmid replication initiator RepA	-
CPZ02_RS25920	88116..88190	-	75	WP_001365705.1	RepA leader peptide Tap	-
CPZ02_RS25930	88424..88681	-	258	WP_000083826.1	replication regulatory protein RepA	-
CPZ02_RS25935	88920..89510	-	591	WP_060710373.1	DUF2726 domain-containing protein	-
CPZ02_RS25940	89548..89757	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
CPZ02_RS25945	89803..90264	-	462	WP_001233857.1	thermonuclease family protein	-
CPZ02_RS25950	90509..90721	-	213	WP_001371875.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / espC / iutA / iucD / iucC / iucB / iucA	1..168972	168972
	-	inside	IScluster/Tn	-	-	83498..87199	3701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10763.47 Da
• Isoelectric Point: 9.9324

>T31218 WP_001620845.1 NZ_AP017611:c85530-85255 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREIRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T31218 NZ_AP017611:c85530-85255 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAGCCCAGGGAGATCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT31218 WP_001178089.1 NZ_AP017611:c85814-85530 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT31218 NZ_AP017611:c85814-85530 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A731IHA8