Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 50686..51208 Replicon plasmid pMRY16-002_1
Accession NZ_AP017611
Organism Escherichia coli strain 20Ec-P-124

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag CPZ02_RS25720 Protein ID WP_000220560.1
Coordinates 50686..50967 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag CPZ02_RS25725 Protein ID WP_000121743.1
Coordinates 50957..51208 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ02_RS25685 47107..47709 - 603 Protein_37 IS3-like element IS2 family transposase -
CPZ02_RS25690 47791..48156 + 366 WP_000124086.1 hypothetical protein -
CPZ02_RS25695 48156..49333 + 1178 Protein_39 IS91 family transposase -
CPZ02_RS25705 49722..49937 - 216 WP_001180116.1 hypothetical protein -
CPZ02_RS25710 49927..50172 - 246 WP_000356546.1 hypothetical protein -
CPZ02_RS25715 50217..50540 - 324 WP_001181903.1 hypothetical protein -
CPZ02_RS25720 50686..50967 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ02_RS25725 50957..51208 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
CPZ02_RS25735 52261..53115 + 855 WP_001239324.1 replication initiation protein -
CPZ02_RS25740 53140..53586 + 447 WP_021532249.1 hypothetical protein -
CPZ02_RS25745 53654..54079 + 426 WP_182585739.1 DNA distortion polypeptide 3 -
CPZ02_RS29165 54131..54364 - 234 WP_024252801.1 YcxB family protein -
CPZ02_RS29170 54542..54727 - 186 Protein_49 YcxB family protein -
CPZ02_RS25760 55162..55446 - 285 WP_001185480.1 hypothetical protein -
CPZ02_RS25765 55546..56208 - 663 WP_000516402.1 peptidyl-arginine deiminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / espC / iutA / iucD / iucC / iucB / iucA 1..168972 168972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T31217 WP_000220560.1 NZ_AP017611:c50967-50686 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T31217 NZ_AP017611:c50967-50686 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT31217 WP_000121743.1 NZ_AP017611:c51208-50957 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT31217 NZ_AP017611:c51208-50957 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References