Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4663759..4664256 Replicon chromosome
Accession NZ_AP017610
Organism Escherichia coli strain 20Ec-P-124

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CPZ02_RS24085 Protein ID WP_001173343.1
Coordinates 4664083..4664256 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag CPZ02_RS24080 Protein ID WP_001317561.1
Coordinates 4663759..4664055 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ02_RS24045 4659419..4660138 - 720 WP_000611288.1 two-component system response regulator DcuR -
CPZ02_RS24050 4660135..4660515 - 381 Protein_4392 GHKL domain-containing protein -
CPZ02_RS24055 4660602..4661573 + 972 WP_170386723.1 IS30-like element ISApl1 family transposase -
CPZ02_RS24060 4661582..4662838 - 1257 Protein_4394 DcuS/MalK family sensor histidine kinase -
CPZ02_RS24065 4663018..4663248 + 231 WP_000371717.1 (4Fe-4S)-binding protein -
CPZ02_RS24070 4663260..4663532 + 273 WP_000405647.1 N-acetyltransferase -
CPZ02_RS24080 4663759..4664055 + 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CPZ02_RS24085 4664083..4664256 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CPZ02_RS24090 4664375..4665892 - 1518 WP_000003805.1 lysine--tRNA ligase -
CPZ02_RS24095 4666129..4667586 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4660650..4673110 12460
- flank IS/Tn - - 4660650..4661573 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T31216 WP_001173343.1 NZ_AP017610:4664083-4664256 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T31216 NZ_AP017610:4664083-4664256 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT31216 WP_001317561.1 NZ_AP017610:4663759-4664055 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT31216 NZ_AP017610:4663759-4664055 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References