Detailed information of TA system TA031207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3857144..3857775
• Replicon: chromosome
• Accession: NZ_AP017610
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: CPZ02_RS20000
• Protein ID: WP_001260301.1
• Coordinates: 3857500..3857775 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: CPZ02_RS19995
• Protein ID: WP_000593557.1
• Coordinates: 3857144..3857503 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS19970	3853276..3854220	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
CPZ02_RS19975	3854217..3855050	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
CPZ02_RS19980	3855050..3855814	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
CPZ02_RS19985	3855811..3856617	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
CPZ02_RS19990	3856623..3857024	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
CPZ02_RS19995	3857144..3857503	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CPZ02_RS20000	3857500..3857775	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
CPZ02_RS20005	3857848..3858972	-	1125	WP_001318088.1	ABC transporter permease	-
CPZ02_RS20010	3858972..3861707	-	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
CPZ02_RS20015	3861704..3862771	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T31207 WP_001260301.1 NZ_AP017610:c3857775-3857500 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T31207 NZ_AP017610:c3857775-3857500 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT31207 WP_000593557.1 NZ_AP017610:c3857503-3857144 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT31207 NZ_AP017610:c3857503-3857144 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1