Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2726717..2727342 Replicon chromosome
Accession NZ_AP017610
Organism Escherichia coli strain 20Ec-P-124

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CPZ02_RS14250 Protein ID WP_000911329.1
Coordinates 2726717..2727115 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CPZ02_RS14255 Protein ID WP_000450524.1
Coordinates 2727115..2727342 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ02_RS14230 2722624..2722824 + 201 WP_000383836.1 YpfN family protein -
CPZ02_RS14235 2722905..2723603 - 699 WP_000679819.1 esterase -
CPZ02_RS14240 2723677..2725692 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
CPZ02_RS14245 2725707..2726570 - 864 WP_001267498.1 neutral zinc metallopeptidase -
CPZ02_RS14250 2726717..2727115 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CPZ02_RS14255 2727115..2727342 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CPZ02_RS14260 2727499..2728212 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CPZ02_RS14265 2728425..2729459 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CPZ02_RS14270 2729476..2730354 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CPZ02_RS14275 2730500..2731072 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CPZ02_RS14280 2731072..2731542 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T31202 WP_000911329.1 NZ_AP017610:c2727115-2726717 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T31202 NZ_AP017610:c2727115-2726717 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT31202 WP_000450524.1 NZ_AP017610:c2727342-2727115 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT31202 NZ_AP017610:c2727342-2727115 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References