Detailed information of TA system TA031192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1568114..1568750
• Replicon: chromosome
• Accession: NZ_AP017610
• Organism: Escherichia coli strain 20Ec-P-124

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: CPZ02_RS08225
• Protein ID: WP_000813796.1
• Coordinates: 1568114..1568290 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CPZ02_RS08230
• Protein ID: WP_076838470.1
• Coordinates: 1568334..1568750 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ02_RS08205	1563736..1564947	-	1212	WP_071593090.1	BenE family transporter YdcO	-
CPZ02_RS08210	1565000..1565536	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
CPZ02_RS08215	1565609..1567570	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CPZ02_RS08220	1567662..1567892	-	231	WP_000494243.1	YncJ family protein	-
CPZ02_RS08225	1568114..1568290	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CPZ02_RS08230	1568334..1568750	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CPZ02_RS08235	1568829..1570235	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
CPZ02_RS08240	1570480..1571625	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
CPZ02_RS08245	1571643..1572656	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
CPZ02_RS08250	1572657..1573598	+	942	WP_001251308.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T31192 WP_000813796.1 NZ_AP017610:1568114-1568290 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T31192 NZ_AP017610:1568114-1568290 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT31192 WP_076838470.1 NZ_AP017610:1568334-1568750 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT31192 NZ_AP017610:1568334-1568750 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3