Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 548501..549119 Replicon chromosome
Accession NZ_AP017610
Organism Escherichia coli strain 20Ec-P-124

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CPZ02_RS02720 Protein ID WP_001291435.1
Coordinates 548501..548719 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CPZ02_RS02725 Protein ID WP_000344800.1
Coordinates 548745..549119 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ02_RS02685 543790..544362 + 573 WP_000779821.1 YbaY family lipoprotein -
CPZ02_RS02690 544393..544704 - 312 WP_000409911.1 MGMT family protein -
CPZ02_RS02700 545083..545436 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CPZ02_RS02705 545478..547028 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CPZ02_RS02710 547192..547662 - 471 WP_000136192.1 YlaC family protein -
CPZ02_RS02715 547778..548329 - 552 WP_000102564.1 maltose O-acetyltransferase -
CPZ02_RS02720 548501..548719 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CPZ02_RS02725 548745..549119 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CPZ02_RS02730 549665..552814 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CPZ02_RS02735 552837..554030 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T31188 WP_001291435.1 NZ_AP017610:c548719-548501 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T31188 NZ_AP017610:c548719-548501 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT31188 WP_000344800.1 NZ_AP017610:c549119-548745 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT31188 NZ_AP017610:c549119-548745 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References