Detailed information of TA system TA031161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/Txe-RelB
• Location: 126278..126785
• Replicon: chromosome
• Accession: NZ_AP017572
• Organism: Rickettsia japonica strain DT-1

Toxin (Protein)

• Gene name: relB
• Uniprot ID: G4KM08
• Locus tag: BZI87_RS00760
• Protein ID: WP_014013837.1
• Coordinates: 126278..126541 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: G4KM09
• Locus tag: BZI87_RS00765
• Protein ID: WP_014013838.1
• Coordinates: 126534..126785 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZI87_RS08080	121372..121491	+	120	WP_158309284.1	palindromic element RPE4 domain-containing protein	-
BZI87_RS00735	121779..122165	+	387	Protein_136	hypothetical protein	-
BZI87_RS00740	122244..122513	+	270	WP_096368100.1	hypothetical protein	-
BZI87_RS08085	122535..122711	+	177	WP_158309285.1	hypothetical protein	-
BZI87_RS00745	122815..124209	-	1395	WP_014120498.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
BZI87_RS00750	124206..124862	-	657	WP_014013835.1	lysophospholipid acyltransferase family protein	-
BZI87_RS00755	125013..126218	-	1206	WP_014120499.1	pyridoxal phosphate-dependent aminotransferase	-
BZI87_RS00760	126278..126541	-	264	WP_014013837.1	Txe/YoeB family addiction module toxin	Toxin
BZI87_RS00765	126534..126785	-	252	WP_014013838.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BZI87_RS00770	126925..127929	+	1005	WP_014120500.1	methyltransferase domain-containing protein	-
BZI87_RS00775	127934..128689	+	756	WP_024704441.1	VacJ family lipoprotein	-
BZI87_RS00780	128709..129308	+	600	WP_014120502.1	phospholipid-binding protein MlaC	-
BZI87_RS00790	129533..129820	+	288	WP_024704442.1	ribose-phosphate pyrophosphokinase-like domain-containing protein	-
BZI87_RS08615	130007..130396	+	390	WP_197535037.1	palindromic element RPE1 domain-containing protein	-
BZI87_RS00800	130541..131608	+	1068	WP_014120503.1	alanine racemase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10590.39 Da
• Isoelectric Point: 9.8255

>T31161 WP_014013837.1 NZ_AP017572:c126541-126278 [Rickettsia japonica]
MIEIEWTLEAAEDLVYWKKYDIKKYERIKLLIKNIQEASFTGIGKPEPLKHILSGLWSRRINHEHRLIYSVNTKQIIIYN
CRFHYKN

Nucleotide

• Length: 264 bp

>T31161 NZ_AP017572:c126541-126278 [Rickettsia japonica]
ATGATTGAGATTGAGTGGACACTAGAAGCAGCGGAGGATTTAGTGTATTGGAAAAAATACGATATTAAAAAATATGAACG
TATTAAACTTCTAATAAAAAATATCCAAGAAGCATCGTTTACAGGTATAGGTAAGCCCGAACCTTTAAAACATATATTAT
CAGGTTTATGGTCACGTAGAATTAACCACGAACATAGACTAATATATTCTGTCAATACTAAACAAATTATAATATATAAT
TGTAGATTTCATTATAAGAATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9537.81 Da
• Isoelectric Point: 9.4884

>AT31161 WP_014013838.1 NZ_AP017572:c126785-126534 [Rickettsia japonica]
MNSISGTSFRKNLSSVLNTVENDHVPYLIKRKNHKNIILLTEEEYESTKETLYLLSNLANANRIKKSIEQAKRGEFAKVN
LDD

Nucleotide

• Length: 252 bp

>AT31161 NZ_AP017572:c126785-126534 [Rickettsia japonica]
ATGAACTCAATTAGCGGCACTTCATTTAGAAAAAACTTAAGCTCTGTACTAAATACCGTAGAAAACGATCATGTCCCTTA
TCTTATTAAAAGAAAGAATCATAAGAATATTATTCTTTTAACCGAAGAAGAATATGAATCTACAAAAGAAACATTATATT
TATTATCTAATCTGGCTAATGCGAATCGAATAAAAAAATCTATCGAACAAGCAAAAAGAGGGGAGTTTGCTAAAGTTAAT
TTAGATGATTGA

Structures

Toxin

• AlphaFold DB: A0A0F3RG12

Antitoxin

• AlphaFold DB: A0A6H3RZ38