Detailed information of TA system TA031115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2212107..2212636
• Replicon: chromosome
• Accession: NZ_AP017377
• Organism: Staphylococcus aureus strain OC8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ATE38_RS11585
• Protein ID: WP_000621175.1
• Coordinates: 2212107..2212469 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ATE38_RS11590
• Protein ID: WP_000948331.1
• Coordinates: 2212466..2212636 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATE38_RS11560	2209085..2209855	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
ATE38_RS11565	2209830..2210309	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
ATE38_RS11570	2210311..2210637	-	327	WP_001052491.1	anti-sigma factor antagonist	-
ATE38_RS11575	2210756..2211757	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ATE38_RS11585	2212107..2212469	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ATE38_RS11590	2212466..2212636	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ATE38_RS11595	2212721..2213869	-	1149	WP_001281145.1	alanine racemase	-
ATE38_RS11600	2213935..2214294	-	360	WP_000581200.1	holo-ACP synthase	-
ATE38_RS11605	2214298..2214789	-	492	WP_001205910.1	PH domain-containing protein	-
ATE38_RS11610	2214776..2216359	-	1584	WP_001294626.1	PH domain-containing protein	-
ATE38_RS11615	2216352..2216831	-	480	WP_001287088.1	hypothetical protein	-
ATE38_RS11620	2217039..2217599	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T31115 WP_000621175.1 NZ_AP017377:c2212469-2212107 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T31115 NZ_AP017377:c2212469-2212107 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT31115 WP_000948331.1 NZ_AP017377:c2212636-2212466 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT31115 NZ_AP017377:c2212636-2212466 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1