Detailed information of TA system TA031104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 93827..94445
• Replicon: plasmid plasmid1 DNA
• Accession: NZ_AP017376
• Organism: Stanieria sp. NIES-3757

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9XZS7
• Locus tag: STA3757_RS24350
• Protein ID: WP_015211942.1
• Coordinates: 94098..94445 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9Y216
• Locus tag: STA3757_RS24345
• Protein ID: WP_015211941.1
• Coordinates: 93827..94093 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STA3757_RS24335	90537..91469	+	933	WP_096388342.1	hypothetical protein	-
STA3757_RS24340	91808..93733	+	1926	WP_096388344.1	DNA-directed DNA polymerase	-
STA3757_RS24345	93827..94093	+	267	WP_015211941.1	CopG family protein	Antitoxin
STA3757_RS24350	94098..94445	+	348	WP_015211942.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
STA3757_RS24355	94472..95452	-	981	WP_096388346.1	hypothetical protein	-
STA3757_RS24360	95528..95809	+	282	WP_015211944.1	hypothetical protein	-
STA3757_RS24365	96037..96693	-	657	WP_096388348.1	hypothetical protein	-
STA3757_RS24370	96783..97124	-	342	WP_096388429.1	hypothetical protein	-
STA3757_RS24375	97165..98913	-	1749	WP_096388350.1	VWA domain-containing protein	-
STA3757_RS24380	99043..99399	-	357	WP_096388352.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..143540	143540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13178.45 Da
• Isoelectric Point: 10.6786

>T31104 WP_015211942.1 NZ_AP017376:94098-94445 [Stanieria sp. NIES-3757]
MSSPVYRQLEIRWIDLEPTRGAETQKKRPCVIIQADIVNQGSKTIIIAPILPGHKNWPFAVNVKPSKSNGLDKERHINLK
QMRAVDVSRIQNRQGILEQQYLKPLQEAIKIIFAL

Nucleotide

• Length: 348 bp

>T31104 NZ_AP017376:94098-94445 [Stanieria sp. NIES-3757]
ATGAGTTCTCCTGTTTATCGGCAATTGGAAATAAGATGGATCGATCTCGAGCCGACAAGGGGTGCTGAAACTCAAAAAAA
AAGACCATGCGTTATTATCCAAGCCGACATTGTTAACCAGGGGTCGAAGACAATTATTATTGCTCCTATTCTTCCAGGAC
ATAAAAATTGGCCTTTTGCAGTCAATGTCAAACCTAGCAAGAGTAACGGATTAGATAAAGAACGACATATCAACCTCAAG
CAGATGAGAGCAGTTGATGTTTCCCGCATCCAAAATCGCCAAGGAATTCTGGAACAGCAATATTTGAAACCGCTTCAGGA
AGCTATTAAGATAATTTTCGCGCTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.02 Da
• Isoelectric Point: 4.2577

>AT31104 WP_015211941.1 NZ_AP017376:93827-94093 [Stanieria sp. NIES-3757]
MTHRATITLDDDAYAFLEASAGNNRSAYINKLLKEEKQRVLAKQIMRANQEEASDLAYQEELMNWDVTLSDGLNERDTAV
SAACPIEL

Nucleotide

• Length: 267 bp

>AT31104 NZ_AP017376:93827-94093 [Stanieria sp. NIES-3757]
ATGACCCATCGTGCAACTATTACTCTAGATGATGATGCCTACGCCTTTTTAGAAGCTTCAGCGGGGAATAATCGTAGTGC
TTATATCAATAAGCTGCTTAAAGAAGAAAAACAACGAGTATTGGCAAAACAGATAATGAGAGCCAATCAAGAAGAAGCTT
CTGACCTTGCTTATCAAGAAGAGCTAATGAATTGGGATGTAACATTGTCTGATGGATTGAACGAGAGGGACACTGCGGTT
TCCGCAGCTTGTCCAATCGAGTTATAA

Structures

Toxin

• AlphaFold DB: K9XZS7

Antitoxin

• AlphaFold DB: K9Y216