Detailed information of TA system TA031103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 76516..77149
• Replicon: plasmid plasmid1 DNA
• Accession: NZ_AP017376
• Organism: Stanieria sp. NIES-3757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: STA3757_RS24245
• Protein ID: WP_096388320.1
• Coordinates: 76754..77149 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9Y1V0
• Locus tag: STA3757_RS24240
• Protein ID: WP_015211880.1
• Coordinates: 76516..76764 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STA3757_RS24225	73517..74149	-	633	WP_096388314.1	tyrosine-type recombinase/integrase	-
STA3757_RS24230	74220..75623	-	1404	WP_096388316.1	ISKra4 family transposase	-
STA3757_RS24235	75620..76294	-	675	WP_096388318.1	plasmid pRiA4b ORF-3 family protein	-
STA3757_RS24240	76516..76764	+	249	WP_015211880.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
STA3757_RS24245	76754..77149	+	396	WP_096388320.1	type II toxin-antitoxin system VapC family toxin	Toxin
STA3757_RS24250	77286..77543	+	258	Protein_74	hypothetical protein	-
STA3757_RS24255	77543..78034	+	492	WP_096388322.1	hypothetical protein	-
STA3757_RS24260	78060..78701	+	642	WP_096388324.1	hypothetical protein	-
STA3757_RS24265	78879..79832	+	954	WP_096388326.1	tyrosine-type recombinase/integrase	-
STA3757_RS24270	79965..80780	+	816	WP_096388328.1	hypothetical protein	-
STA3757_RS24275	80788..81294	-	507	WP_096383530.1	transposase	-
STA3757_RS24280	81260..81607	-	348	WP_096387865.1	transposase	-
STA3757_RS24285	81696..81947	-	252	WP_071882437.1	WGR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..143540	143540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14361.42 Da
• Isoelectric Point: 4.4076

>T31103 WP_096388320.1 NZ_AP017376:76754-77149 [Stanieria sp. NIES-3757]
MVIDTSAIIAILNLEPEATILADGIASDSVRLMSMGTALELSIIVKARKEEAGIRELDFFLYKAAINLVNFDENQLKIAR
YAFENYGKGRNPASLNFGDCFAYALSKTSGQPLLFKGNDFNQTDVSVAVEF

Nucleotide

• Length: 396 bp

>T31103 NZ_AP017376:76754-77149 [Stanieria sp. NIES-3757]
ATGGTTATTGATACGAGTGCGATTATAGCGATTCTTAATCTTGAACCTGAAGCAACTATCTTAGCTGATGGAATCGCTTC
GGACTCAGTTCGTCTTATGTCTATGGGAACTGCCTTAGAACTATCAATAATAGTTAAGGCGAGAAAAGAAGAAGCTGGAA
TTAGAGAATTAGATTTCTTTTTATATAAAGCAGCCATTAATTTAGTCAATTTTGATGAAAATCAACTAAAAATAGCTCGT
TATGCTTTTGAGAATTATGGTAAAGGCAGAAATCCCGCCAGTTTAAATTTTGGTGATTGTTTTGCTTATGCTCTTAGTAA
AACTTCTGGACAACCCTTATTGTTCAAAGGTAATGATTTTAACCAAACTGATGTTTCAGTAGCAGTTGAATTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9427.65 Da
• Isoelectric Point: 4.3822

>AT31103 WP_015211880.1 NZ_AP017376:76516-76764 [Stanieria sp. NIES-3757]
MAINIKNVEADRLARELSEITGETITEAVIKSLAERLEREKNKQITPLPLQQELLNIAQRYQTLPTLDNRTEEEILGYND
GY

Nucleotide

• Length: 249 bp

>AT31103 NZ_AP017376:76516-76764 [Stanieria sp. NIES-3757]
ATGGCGATTAATATTAAAAACGTTGAAGCAGATAGACTGGCTAGGGAATTAAGCGAAATAACTGGAGAAACAATCACAGA
AGCTGTAATTAAATCTTTGGCTGAAAGACTAGAAAGGGAAAAAAATAAACAAATTACTCCTTTGCCTTTACAACAAGAAC
TACTAAATATTGCTCAACGGTATCAAACCCTGCCGACTTTAGATAACCGAACAGAGGAAGAAATTTTGGGTTACAACGAT
GGTTATTGA

Structures

Antitoxin

• AlphaFold DB: K9Y1V0