Detailed information of TA system TA031091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3043048..3043640
• Replicon: chromosome
• Accession: NZ_AP017367
• Organism: Leptolyngbya sp. O-77

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: O77CONTIG1_RS12965
• Protein ID: WP_068511186.1
• Coordinates: 3043263..3043640 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: O77CONTIG1_RS12960
• Protein ID: WP_068511184.1
• Coordinates: 3043048..3043263 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O77CONTIG1_RS12950	3038570..3039676	-	1107	WP_068511180.1	glutamate 5-kinase	-
O77CONTIG1_RS12955	3040434..3042704	+	2271	WP_156435236.1	hypothetical protein	-
O77CONTIG1_RS12960	3043048..3043263	+	216	WP_068511184.1	DUF2281 domain-containing protein	Antitoxin
O77CONTIG1_RS12965	3043263..3043640	+	378	WP_068511186.1	type II toxin-antitoxin system VapC family toxin	Toxin
O77CONTIG1_RS12970	3043786..3045081	+	1296	WP_084782593.1	AI-2E family transporter	-
O77CONTIG1_RS12975	3045249..3045608	+	360	WP_068511188.1	hypothetical protein	-
O77CONTIG1_RS12980	3045643..3046511	+	869	Protein_2637	branched-chain amino acid ABC transporter permease	-
O77CONTIG1_RS12985	3046663..3047999	-	1337	Protein_2638	1-acyl-sn-glycerol-3-phosphate acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (2-124)

Antitoxin

• Domain of unknown function DUF2281 (6-70)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14414.94 Da
• Isoelectric Point: 4.6779

>T31091 WP_068511186.1 NZ_AP017367:3043263-3043640 [Leptolyngbya sp. O-77]
MLFDTHVLLWFLEDDPRLPPQIKQMIEVSNDVFVSIIALLEVAIKISVGKLELQLEFQKLTGFLADLDIKILTISFEDIK
RYVDLPLCHRDPFDRMLIAQSINHAMSIVSADSAFDAYPIRRLWA

Nucleotide

• Length: 378 bp

>T31091 NZ_AP017367:3043263-3043640 [Leptolyngbya sp. O-77]
ATGCTATTCGATACTCATGTCCTTCTTTGGTTTCTAGAAGATGATCCCAGGCTGCCTCCACAAATTAAGCAGATGATTGA
GGTCAGCAATGATGTTTTTGTTAGCATTATTGCCCTATTGGAAGTCGCTATAAAAATTTCTGTTGGTAAACTGGAATTAC
AACTTGAGTTTCAAAAACTAACAGGTTTTCTAGCCGATCTTGATATTAAAATTCTGACGATTTCTTTTGAAGACATCAAG
CGATATGTTGATCTACCGCTTTGCCATCGAGATCCGTTTGATCGGATGCTGATTGCCCAATCCATTAACCACGCTATGAG
TATTGTCAGCGCTGATTCGGCGTTTGATGCGTATCCGATTCGGCGGTTGTGGGCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8270.73 Da
• Isoelectric Point: 4.5859

>AT31091 WP_068511184.1 NZ_AP017367:3043048-3043263 [Leptolyngbya sp. O-77]
MLQPAIVEKLEKLPEPLQTEVLHYIEFLLDKYVPDVSVQVEPKKRKAGLLKGKIWMADDFDAPLEDLKDYM

Nucleotide

• Length: 216 bp

>AT31091 NZ_AP017367:3043048-3043263 [Leptolyngbya sp. O-77]
ATGCTCCAGCCAGCGATTGTAGAAAAGCTAGAGAAATTACCTGAACCTTTGCAAACAGAAGTCCTGCACTATATCGAGTT
CTTGCTAGATAAATACGTCCCGGACGTGTCTGTGCAGGTTGAACCTAAGAAACGCAAAGCAGGTTTATTGAAAGGGAAGA
TTTGGATGGCAGATGATTTCGACGCTCCTCTTGAGGACTTAAAGGATTATATGTAA