Detailed information of TA system TA031081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180630..2181159
• Replicon: chromosome
• Accession: NZ_AP017320
• Organism: Staphylococcus aureus strain MI

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAMI_RS11150
• Protein ID: WP_000621175.1
• Coordinates: 2180630..2180992 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAMI_RS11155
• Protein ID: WP_000948331.1
• Coordinates: 2180989..2181159 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMI_RS11125	2177608..2178378	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
SAMI_RS11130	2178353..2178832	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAMI_RS11135	2178834..2179160	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAMI_RS11140	2179279..2180280	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAMI_RS11150	2180630..2180992	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAMI_RS11155	2180989..2181159	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAMI_RS11160	2181244..2182392	-	1149	WP_001281154.1	alanine racemase	-
SAMI_RS11165	2182458..2182817	-	360	WP_000581197.1	holo-ACP synthase	-
SAMI_RS11170	2182821..2183312	-	492	WP_001205912.1	PH domain-containing protein	-
SAMI_RS11175	2183299..2184882	-	1584	WP_001294626.1	PH domain-containing protein	-
SAMI_RS11180	2184875..2185354	-	480	WP_001287087.1	hypothetical protein	-
SAMI_RS11185	2185562..2186122	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T31081 WP_000621175.1 NZ_AP017320:c2180992-2180630 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T31081 NZ_AP017320:c2180992-2180630 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT31081 WP_000948331.1 NZ_AP017320:c2181159-2180989 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT31081 NZ_AP017320:c2181159-2180989 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1