Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 6198218..6198798 Replicon chromosome
Accession NZ_AP017308
Organism Leptolyngbya sp. NIES-3755

Toxin (Protein)


Gene name mazF Uniprot ID A0A0S3UE13
Locus tag LEP3755_RS30410 Protein ID WP_068392319.1
Coordinates 6198218..6198481 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0S3UDS3
Locus tag LEP3755_RS30415 Protein ID WP_068392321.1
Coordinates 6198469..6198798 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LEP3755_RS30385 6193311..6194600 - 1290 WP_068392307.1 hypothetical protein -
LEP3755_RS30390 6194853..6196487 - 1635 WP_068392310.1 alpha-D-glucose phosphate-specific phosphoglucomutase -
LEP3755_RS30395 6196644..6197108 + 465 WP_068392313.1 hypothetical protein -
LEP3755_RS30400 6197111..6197686 + 576 WP_068392316.1 glutathione S-transferase family protein -
LEP3755_RS30405 6197955..6198092 - 138 WP_017289085.1 photosystem II reaction center protein K -
LEP3755_RS30410 6198218..6198481 + 264 WP_068392319.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
LEP3755_RS30415 6198469..6198798 + 330 WP_068392321.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
LEP3755_RS30420 6198800..6199930 - 1131 WP_084762137.1 tRNA guanosine(34) transglycosylase Tgt -
LEP3755_RS30425 6200135..6200941 + 807 WP_068392332.1 metal ABC transporter ATP-binding protein -
LEP3755_RS30430 6200944..6201849 + 906 WP_068392335.1 metal ABC transporter permease -
LEP3755_RS30435 6201827..6202123 - 297 WP_068392338.1 hypothetical protein -
LEP3755_RS30440 6202283..6203776 + 1494 WP_068392343.1 nucleoside:proton symporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(10-49)

Antitoxin

mRNA interferase PemK-like

(3-107)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 9886.05 Da        Isoelectric Point: 4.2808

>T31065 WP_068392319.1 NZ_AP017308:6198218-6198481 [Leptolyngbya sp. NIES-3755]
MSTTIRTQIVKVGNSQGIRIPKRLLEQSGIKGEVEIEVQQNQLIIRPVAHPRAGWEDAIEAVIAEHGDEGLLDDETPTQW
DETEWQW

Download         Length: 264 bp

>T31065 NZ_AP017308:6198218-6198481 [Leptolyngbya sp. NIES-3755]
ATGAGTACAACCATTAGGACTCAAATTGTGAAAGTCGGAAATTCTCAAGGAATCAGAATTCCGAAGCGCCTCCTGGAACA
GAGCGGCATCAAAGGCGAGGTCGAGATTGAAGTTCAGCAGAATCAATTGATTATTCGTCCGGTTGCTCATCCACGGGCAG
GATGGGAAGACGCGATCGAGGCAGTCATCGCAGAACATGGTGACGAAGGATTGCTAGATGATGAAACTCCGACGCAGTGG
GACGAAACCGAATGGCAATGGTAG

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12419.51 Da        Isoelectric Point: 6.2977

>AT31065 WP_068392321.1 NZ_AP017308:6198469-6198798 [Leptolyngbya sp. NIES-3755]
MAMVVRRFDVFLVQLDPTVGFEIQKTRPCVIISPDEMNRSLKTVLIAPLTSVKRNFPFRVDCQFQGKEGQIVLDQIRAVD
KTRLVQLLGQVTSDEQRAILDNLAEIFAE

Download         Length: 330 bp

>AT31065 NZ_AP017308:6198469-6198798 [Leptolyngbya sp. NIES-3755]
ATGGCAATGGTAGTGCGTCGATTTGATGTATTTCTGGTGCAGCTAGATCCGACCGTTGGATTTGAAATTCAGAAAACTCG
TCCCTGTGTGATTATTTCTCCCGACGAGATGAATCGATCGTTAAAGACAGTTCTGATTGCACCGTTGACCTCGGTAAAGC
GAAACTTTCCATTTCGAGTAGACTGCCAGTTTCAAGGAAAAGAAGGGCAAATTGTACTCGATCAAATCCGAGCAGTAGAC
AAAACTCGACTTGTTCAACTTTTAGGTCAAGTGACTTCAGACGAACAAAGAGCAATTCTCGACAACTTAGCTGAAATCTT
TGCAGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S3UE13

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3UDS3

References