Detailed information of TA system TA031062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3056340..3056923
• Replicon: chromosome
• Accession: NZ_AP017308
• Organism: Leptolyngbya sp. NIES-3755

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LEP3755_RS14590
• Protein ID: WP_068384372.1
• Coordinates: 3056340..3056687 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0S3U4Q5
• Locus tag: LEP3755_RS14595
• Protein ID: WP_068384374.1
• Coordinates: 3056681..3056923 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEP3755_RS14560	3051644..3052504	+	861	WP_068384362.1	Rpn family recombination-promoting nuclease/putative transposase	-
LEP3755_RS14565	3052567..3052986	+	420	WP_068384364.1	hypothetical protein	-
LEP3755_RS14570	3052983..3053285	-	303	WP_068393164.1	hypothetical protein	-
LEP3755_RS14575	3053423..3053947	-	525	WP_068384366.1	hypothetical protein	-
LEP3755_RS14580	3054444..3054980	-	537	WP_068384368.1	ribosome maturation factor RimM	-
LEP3755_RS14585	3054986..3056254	-	1269	WP_068384370.1	valine--pyruvate transaminase	-
LEP3755_RS14590	3056340..3056687	-	348	WP_068384372.1	endoribonuclease MazF	Toxin
LEP3755_RS14595	3056681..3056923	-	243	WP_068384374.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LEP3755_RS14600	3056972..3057454	-	483	WP_068393166.1	GNAT family N-acetyltransferase	-
LEP3755_RS14605	3057451..3057633	-	183	WP_068384376.1	hypothetical protein	-
LEP3755_RS14610	3057912..3058865	-	954	WP_068384378.1	isoaspartyl peptidase/L-asparaginase	-
LEP3755_RS33280	3058917..3059081	-	165	WP_071837130.1	DUF2256 domain-containing protein	-
LEP3755_RS14615	3059375..3060310	+	936	WP_071837131.1	trypsin-like peptidase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-113)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13084.14 Da
• Isoelectric Point: 9.5103

>T31062 WP_068384372.1 NZ_AP017308:c3056687-3056340 [Leptolyngbya sp. NIES-3755]
MVKRSNPHIPDRGDIIYLDFDPTIGREQKGARPGFVLSPRSYNQKSSLALVMPITKHQKGYPFEVLLPDGLKTHGVILTD
QIKSIDWRGRNVQFVESVAEEIIEEIQAKIRALLF

Nucleotide

• Length: 348 bp

>T31062 NZ_AP017308:c3056687-3056340 [Leptolyngbya sp. NIES-3755]
TTGGTAAAGCGATCGAATCCTCATATTCCCGATCGTGGAGACATCATCTATCTCGACTTTGACCCCACGATCGGGCGCGA
ACAGAAAGGAGCTAGACCAGGGTTTGTCCTTTCGCCACGGTCTTACAATCAAAAATCTTCCCTTGCGTTAGTGATGCCAA
TTACGAAACACCAAAAGGGATATCCGTTTGAGGTGCTTCTGCCTGATGGACTCAAAACGCATGGTGTGATTCTGACTGAC
CAAATTAAATCTATTGATTGGCGAGGACGGAATGTTCAGTTCGTAGAGTCTGTTGCAGAAGAAATTATTGAAGAGATTCA
AGCAAAGATAAGAGCACTCTTGTTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8859.13 Da
• Isoelectric Point: 4.5822

>AT31062 WP_068384374.1 NZ_AP017308:c3056923-3056681 [Leptolyngbya sp. NIES-3755]
MASMVAKWGNSLAIRIPQNLAKELQISEGAEVEISAIDGTLVVKPRKRKEYSLDELLEDITPENLHAAVDFGRPVGNEVW

Nucleotide

• Length: 243 bp

>AT31062 NZ_AP017308:c3056923-3056681 [Leptolyngbya sp. NIES-3755]
ATGGCTTCAATGGTTGCAAAGTGGGGAAACAGTCTTGCGATTCGTATCCCACAAAATTTAGCGAAAGAGCTTCAGATTTC
CGAAGGTGCAGAGGTCGAAATCAGTGCAATTGATGGAACTTTAGTGGTGAAACCGAGAAAGCGCAAAGAGTATTCACTCG
ATGAATTACTAGAAGATATTACGCCAGAAAATCTTCATGCAGCCGTTGACTTCGGCAGACCAGTGGGGAACGAAGTTTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3U4Q5