Detailed information of TA system TA031059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/-
• Location: 711638..712315
• Replicon: chromosome
• Accession: NZ_AP017308
• Organism: Leptolyngbya sp. NIES-3755

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LEP3755_RS03300
• Protein ID: WP_068380408.1
• Coordinates: 711974..712315 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S3TY70
• Locus tag: LEP3755_RS03295
• Protein ID: WP_068380406.1
• Coordinates: 711638..711892 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEP3755_RS03265	707049..708098	-	1050	WP_068380387.1	glucokinase	-
LEP3755_RS03270	708178..708822	+	645	WP_068380390.1	pyridoxamine 5'-phosphate oxidase	-
LEP3755_RS03275	708835..709071	+	237	WP_068380393.1	hypothetical protein	-
LEP3755_RS03280	709059..709391	+	333	WP_068380397.1	XisI protein	-
LEP3755_RS03285	709468..709851	+	384	WP_068380399.1	hypothetical protein	-
LEP3755_RS03290	709919..711541	+	1623	WP_068380404.1	DUF3352 domain-containing protein	-
LEP3755_RS03295	711638..711892	-	255	WP_068380406.1	hypothetical protein	Antitoxin
LEP3755_RS03300	711974..712315	-	342	WP_068380408.1	addiction module antitoxin	Toxin
LEP3755_RS03305	712555..712764	-	210	WP_068380411.1	hypothetical protein	-
LEP3755_RS03310	713203..713433	-	231	WP_068380413.1	hypothetical protein	-
LEP3755_RS03315	713420..713680	-	261	WP_068380414.1	hypothetical protein	-
LEP3755_RS03320	713814..716249	-	2436	WP_068380417.1	toll/interleukin-1 receptor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12876.76 Da
• Isoelectric Point: 9.3081

>T31059 WP_068380408.1 NZ_AP017308:c712315-711974 [Leptolyngbya sp. NIES-3755]
IQIDLTPECKQNLKELAKRYRSIRSDLQEVLGSLQAGDFIGDRLTGIGKGYIVLKARVKNRNLQKGKSGGYHLIYQIESS
SSILLLTIYAKSDREDITVQEVRDILKEFYQTD

Nucleotide

• Length: 342 bp

>T31059 NZ_AP017308:c712315-711974 [Leptolyngbya sp. NIES-3755]
ATTCAGATAGATTTAACTCCTGAATGTAAGCAAAATCTGAAAGAACTCGCAAAGCGTTATCGGAGTATTCGCTCTGATTT
GCAAGAAGTGTTGGGATCGCTGCAAGCAGGTGACTTTATTGGCGATCGATTAACTGGAATCGGCAAAGGGTATATTGTCC
TCAAAGCAAGAGTCAAAAACCGAAATCTTCAGAAGGGCAAAAGCGGCGGCTATCACCTGATCTATCAAATCGAATCATCC
TCGTCGATTCTACTACTCACCATTTATGCAAAGTCCGATCGCGAAGATATTACCGTACAAGAAGTGCGAGATATTCTGAA
AGAGTTTTATCAGACCGATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9255.37 Da
• Isoelectric Point: 3.9370

>AT31059 WP_068380406.1 NZ_AP017308:c711892-711638 [Leptolyngbya sp. NIES-3755]
MRAVEVTGMVDEQGNLSLNEPLPTAAPGEVRIIILYPDQAETPEQDPDDTPVKEIKASLRRALQQATSGQRIPLDQMWEG
IDAE

Nucleotide

• Length: 255 bp

>AT31059 NZ_AP017308:c711892-711638 [Leptolyngbya sp. NIES-3755]
ATGAGAGCAGTCGAAGTAACAGGCATGGTCGATGAACAGGGAAATCTCTCTCTGAATGAGCCATTACCGACCGCTGCTCC
TGGCGAGGTGCGAATCATTATTTTGTACCCAGATCAGGCAGAAACACCAGAGCAAGACCCTGACGATACACCCGTCAAAG
AGATCAAAGCCAGTCTACGTAGAGCCTTGCAGCAAGCCACTTCAGGGCAAAGAATTCCACTTGATCAAATGTGGGAAGGA
ATTGATGCGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3TY70