Detailed information of TA system TA031048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 4916998..4917436
• Replicon: chromosome
• Accession: NZ_AP017295
• Organism: Nostoc sp. NIES-3756

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: NOS3756_RS29850
• Protein ID: WP_082727292.1
• Coordinates: 4917254..4917436 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: NOS3756_RS20390
• Protein ID: WP_067771832.1
• Coordinates: 4916998..4917207 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOS3756_RS20370	4912641..4913897	-	1257	WP_067771819.1	50S ribosome-binding GTPase	-
NOS3756_RS20375	4913933..4914979	-	1047	WP_067771822.1	DUF1611 domain-containing protein	-
NOS3756_RS20380	4914963..4916015	-	1053	WP_067771826.1	dipeptide epimerase	-
NOS3756_RS20385	4916153..4916707	+	555	WP_067771829.1	NUDIX hydrolase	-
NOS3756_RS29845	4916783..4916944	+	162	WP_082727381.1	type II toxin-antitoxin system HicB family antitoxin	-
NOS3756_RS20390	4916998..4917207	+	210	WP_067771832.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NOS3756_RS29850	4917254..4917436	+	183	WP_082727292.1	type II toxin-antitoxin system HicA family toxin	Toxin
NOS3756_RS20395	4917470..4919233	-	1764	WP_082727382.1	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase RibB/GTP cyclohydrolase II RibA	-
NOS3756_RS20400	4919482..4920540	+	1059	WP_067771835.1	N-acetyl-gamma-glutamyl-phosphate reductase	-
NOS3756_RS20405	4920656..4921945	-	1290	WP_067771838.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-48)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-51)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6862.72 Da
• Isoelectric Point: 5.5255

>T31048 WP_082727292.1 NZ_AP017295:4917254-4917436 [Nostoc sp. NIES-3756]
ILENLGFVEVQQKGSHKQFRHEDGQGTTVPFHQGWDISPSLLRKIASDIYLTVEELLEAR

Nucleotide

• Length: 183 bp

>T31048 NZ_AP017295:4917254-4917436 [Nostoc sp. NIES-3756]
ATTTTAGAAAACCTCGGCTTTGTGGAGGTTCAGCAAAAAGGCTCACATAAGCAGTTTCGTCATGAGGATGGACAAGGCAC
AACCGTTCCATTTCACCAAGGTTGGGACATATCTCCCAGTTTGCTACGAAAGATAGCCAGCGACATTTATTTAACAGTTG
AGGAACTGTTAGAGGCGAGATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7798.81 Da
• Isoelectric Point: 3.9166

>AT31048 WP_067771832.1 NZ_AP017295:4916998-4917207 [Nostoc sp. NIES-3756]
MKIFTAIIDKDLDTNLYVGYVPGFAGAHSQGETLDELQHNLREVIEMLLEDEEVIFTTQFVGTQQIVVN

Nucleotide

• Length: 210 bp

>AT31048 NZ_AP017295:4916998-4917207 [Nostoc sp. NIES-3756]
ATGAAAATCTTTACTGCTATTATTGATAAAGATTTGGATACAAATCTCTATGTAGGCTATGTCCCTGGATTTGCAGGAGC
GCATTCTCAGGGAGAAACTTTAGATGAGTTACAACACAACCTCCGCGAAGTAATTGAAATGCTTCTGGAAGATGAAGAAG
TAATTTTTACGACACAATTTGTTGGTACACAGCAGATTGTGGTTAACTGA