Detailed information of TA system TA031046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1453528..1454134
• Replicon: chromosome
• Accession: NZ_AP017295
• Organism: Nostoc sp. NIES-3756

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Z4ID51
• Locus tag: NOS3756_RS06080
• Protein ID: WP_067765928.1
• Coordinates: 1453528..1453824 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: NOS3756_RS06085
• Protein ID: WP_067765931.1
• Coordinates: 1453814..1454134 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOS3756_RS31450	1448958..1449272	-	315	WP_067765911.1	50S ribosomal protein L23	-
NOS3756_RS06055	1449265..1449897	-	633	WP_067765914.1	50S ribosomal protein L4	-
NOS3756_RS06060	1449962..1450597	-	636	WP_067765917.1	50S ribosomal protein L3	-
NOS3756_RS06065	1451424..1451912	+	489	WP_067765920.1	NAD(P)H-quinone oxidoreductase subunit N	-
NOS3756_RS06070	1451988..1452464	-	477	WP_067765923.1	GNAT family N-acetyltransferase	-
NOS3756_RS06075	1452507..1453334	-	828	WP_067765925.1	ABC transporter permease	-
NOS3756_RS06080	1453528..1453824	+	297	WP_067765928.1	type II toxin-antitoxin system HicA family toxin	Toxin
NOS3756_RS06085	1453814..1454134	+	321	WP_067765931.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NOS3756_RS06090	1454285..1454833	-	549	WP_067764428.1	ISAs1 family transposase	-
NOS3756_RS06095	1454824..1455378	-	555	WP_067764431.1	ISAs1 family transposase	-
NOS3756_RS06100	1455546..1456001	-	456	WP_067765934.1	pentapeptide repeat-containing protein	-
NOS3756_RS06105	1456313..1457176	-	864	WP_067765937.1	alpha/beta hydrolase	-
NOS3756_RS06110	1457304..1458140	+	837	WP_067765940.1	endonuclease/exonuclease/phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (25-83)

Antitoxin

• Uncharacterised protein family HicB (51-101)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10963.47 Da
• Isoelectric Point: 7.3215

>T31046 WP_067765928.1 NZ_AP017295:1453528-1453824 [Nostoc sp. NIES-3756]
MVLNSKHQKTLDDVFESPVRADILWSDIESLLIALGAEVSEGRGSRVRIALNGVKAIFHRPHPQKETDKGAVKSIRRFLT
EAGVKEDISTRELNNNEI

Nucleotide

• Length: 297 bp

>T31046 NZ_AP017295:1453528-1453824 [Nostoc sp. NIES-3756]
ATGGTACTAAACAGCAAACATCAAAAAACCCTAGATGACGTTTTTGAAAGTCCGGTTAGAGCCGATATCCTGTGGAGTGA
TATTGAGTCTTTGTTGATTGCGCTTGGTGCGGAAGTGTCAGAAGGAAGGGGGTCAAGAGTGAGGATAGCTCTTAATGGTG
TAAAAGCAATTTTTCACCGACCACACCCACAAAAAGAAACTGATAAAGGTGCTGTTAAGTCTATACGGCGCTTTTTAACA
GAAGCTGGGGTTAAAGAAGACATAAGCACAAGGGAGCTAAATAACAATGAGATATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12330.02 Da
• Isoelectric Point: 4.9965

>AT31046 WP_067765931.1 NZ_AP017295:1453814-1454134 [Nostoc sp. NIES-3756]
MRYKGYEAVVEFDDEAEIFHGEVINIRDVITFQADNVKDLKQAFHDSIDDYLEFCKERGEEPEKPFSGKLMLRINPELHK
TIAVKAKKEGKSLNSWIEKCLCQYTG

Nucleotide

• Length: 321 bp

>AT31046 NZ_AP017295:1453814-1454134 [Nostoc sp. NIES-3756]
ATGAGATATAAAGGATATGAAGCTGTTGTTGAGTTTGATGATGAAGCAGAAATTTTTCATGGAGAAGTAATCAACATTCG
AGATGTGATTACATTTCAAGCTGATAACGTAAAAGACTTAAAGCAAGCCTTTCACGACTCAATTGATGATTATTTAGAAT
TTTGCAAAGAACGTGGTGAAGAACCGGAAAAGCCATTTTCAGGTAAACTGATGCTCAGAATTAACCCTGAATTGCATAAA
ACAATTGCTGTTAAAGCAAAGAAAGAGGGTAAAAGCCTTAATTCTTGGATAGAAAAATGCTTGTGTCAGTATACGGGTTA
A

Structures

Toxin

• AlphaFold DB: A0A1Z4ID51