Detailed information of TA system TA031032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 994138..994687
• Replicon: chromosome
• Accession: NZ_AP014946
• Organism: Variibacter gotjawalensis strain GJW-30

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GJW30_RS04770
• Protein ID: WP_096352324.1
• Coordinates: 994430..994687 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GJW30_RS04765
• Protein ID: WP_096352322.1
• Coordinates: 994138..994446 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJW30_RS04740	989175..991742	-	2568	WP_096352308.1	FtsX-like permease family protein	-
GJW30_RS04745	991739..992449	-	711	WP_096352310.1	ABC transporter ATP-binding protein	-
GJW30_RS04750	992499..993188	+	690	WP_096352313.1	arylesterase	-
GJW30_RS04755	993288..993836	+	549	WP_096352316.1	RNA 2',3'-cyclic phosphodiesterase	-
GJW30_RS04760	993742..994131	+	390	WP_096352319.1	4a-hydroxytetrahydrobiopterin dehydratase	-
GJW30_RS04765	994138..994446	-	309	WP_096352322.1	BrnA antitoxin family protein	Antitoxin
GJW30_RS04770	994430..994687	-	258	WP_096352324.1	BrnT family toxin	Toxin
GJW30_RS04775	994823..995176	+	354	WP_096352328.1	DUF1232 domain-containing protein	-
GJW30_RS04780	995277..996647	+	1371	WP_096352331.1	glutamate--cysteine ligase	-
GJW30_RS04785	996815..997393	+	579	WP_096352334.1	LysE family transporter	-
GJW30_RS04790	997395..998828	-	1434	WP_096352337.1	chromate transporter	-
GJW30_RS04795	998761..999123	-	363	WP_096358658.1	metallopeptidase family protein	-
GJW30_RS04800	999216..999449	-	234	WP_096352340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9861.38 Da
• Isoelectric Point: 10.2126

>T31032 WP_096352324.1 NZ_AP014946:c994687-994430 [Variibacter gotjawalensis]
MQIVWDEPKRLKNLLTHRLDFADLRIDFFLTADIRPSYAGRATATGVFESTMITVVFSRLGSEAISLISMRHSSRKERRL
YEKEA

Nucleotide

• Length: 258 bp

>T31032 NZ_AP014946:c994687-994430 [Variibacter gotjawalensis]
ATGCAGATCGTTTGGGACGAGCCGAAGCGGCTCAAGAATTTACTGACCCATAGGCTGGATTTTGCGGACCTGCGGATCGA
TTTTTTCCTTACCGCCGACATCCGGCCGAGCTACGCGGGTCGCGCCACAGCCACCGGCGTGTTTGAGAGCACGATGATCA
CGGTCGTGTTCTCGAGACTCGGGAGCGAGGCGATCTCGCTGATCTCGATGCGCCATTCGAGTAGGAAGGAGCGGAGGCTC
TATGAAAAAGAAGCTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11759.46 Da
• Isoelectric Point: 10.4136

>AT31032 WP_096352322.1 NZ_AP014946:c994446-994138 [Variibacter gotjawalensis]
MKKKLDHLRPLTDAEEARIQRGIAEDPENPEWTEQDFKTARPFAEVFPELAESIRRARGRPPVETPRQQISIRLDPDVIA
KFKATGKGWQSRINDVLKKAKV

Nucleotide

• Length: 309 bp

>AT31032 NZ_AP014946:c994446-994138 [Variibacter gotjawalensis]
ATGAAAAAGAAGCTTGATCATTTGAGGCCTCTCACCGACGCCGAAGAAGCACGCATCCAGCGCGGGATCGCGGAAGATCC
AGAGAATCCCGAGTGGACCGAGCAGGACTTCAAAACCGCGCGCCCATTCGCCGAAGTCTTCCCCGAGCTCGCCGAAAGCA
TCCGGCGCGCGCGCGGGAGGCCACCGGTCGAGACGCCGCGTCAGCAGATTTCGATCCGGCTCGATCCCGACGTCATCGCG
AAATTCAAGGCGACCGGCAAAGGCTGGCAGTCGCGCATCAACGATGTGCTGAAGAAGGCGAAGGTGTAG