Detailed information of TA system TA031022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2103826..2104355
• Replicon: chromosome
• Accession: NZ_AP014942
• Organism: Staphylococcus aureus strain FDA209P

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAFDA_RS10605
• Protein ID: WP_000621175.1
• Coordinates: 2103826..2104188 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAFDA_RS10610
• Protein ID: WP_000948331.1
• Coordinates: 2104185..2104355 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAFDA_RS10580	2100804..2101574	-	771	WP_060829077.1	RNA polymerase sigma factor SigB	-
SAFDA_RS10585	2101549..2102028	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAFDA_RS10590	2102030..2102356	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAFDA_RS10595	2102475..2103476	-	1002	WP_042727554.1	PP2C family protein-serine/threonine phosphatase	-
SAFDA_RS10605	2103826..2104188	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAFDA_RS10610	2104185..2104355	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAFDA_RS10615	2104440..2105588	-	1149	WP_001281154.1	alanine racemase	-
SAFDA_RS10620	2105654..2106013	-	360	WP_000581197.1	holo-ACP synthase	-
SAFDA_RS10625	2106017..2106508	-	492	WP_001205912.1	PH domain-containing protein	-
SAFDA_RS10630	2106495..2108078	-	1584	WP_001294620.1	PH domain-containing protein	-
SAFDA_RS10635	2108071..2108550	-	480	WP_001287079.1	hypothetical protein	-
SAFDA_RS10640	2108759..2109319	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T31022 WP_000621175.1 NZ_AP014942:c2104188-2103826 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T31022 NZ_AP014942:c2104188-2103826 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT31022 WP_000948331.1 NZ_AP014942:c2104355-2104185 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT31022 NZ_AP014942:c2104355-2104185 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1