Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 552418..553054 Replicon chromosome
Accession NZ_AP014928
Organism Bacillus pumilus strain TUAT1

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag BTUAT1_RS02680 Protein ID WP_003214169.1
Coordinates 552704..553054 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag BTUAT1_RS02675 Protein ID WP_003214273.1
Coordinates 552418..552699 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTUAT1_RS02655 548572..549177 - 606 WP_060832102.1 rhomboid family intramembrane serine protease -
BTUAT1_RS02660 549272..549637 + 366 WP_019744212.1 holo-ACP synthase -
BTUAT1_RS02665 549798..550814 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
BTUAT1_RS02670 550943..552124 + 1182 WP_008346911.1 alanine racemase -
BTUAT1_RS02675 552418..552699 + 282 WP_003214273.1 hypothetical protein Antitoxin
BTUAT1_RS02680 552704..553054 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTUAT1_RS02685 553171..554001 + 831 WP_008346908.1 RsbT co-antagonist protein RsbRA -
BTUAT1_RS02690 554006..554374 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
BTUAT1_RS02695 554377..554778 + 402 WP_060699688.1 anti-sigma regulatory factor -
BTUAT1_RS02700 554789..555796 + 1008 WP_017358394.1 PP2C family protein-serine/threonine phosphatase -
BTUAT1_RS02705 555856..556185 + 330 WP_017358393.1 anti-sigma factor antagonist -
BTUAT1_RS02710 556182..556670 + 489 WP_048001251.1 anti-sigma B factor RsbW -
BTUAT1_RS02715 556636..557424 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
BTUAT1_RS02720 557424..558023 + 600 WP_008346897.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T31015 WP_003214169.1 NZ_AP014928:552704-553054 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T31015 NZ_AP014928:552704-553054 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGATACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT31015 WP_003214273.1 NZ_AP014928:552418-552699 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT31015 NZ_AP014928:552418-552699 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTGATGTCTGAAATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References