Detailed information of TA system TA030999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 389073..389584
• Replicon: chromosome
• Accession: NZ_AP014921
• Organism: Staphylococcus aureus strain JH4899

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: -
• Locus tag: JSA_RS01850
• Protein ID: WP_031904328.1
• Coordinates: 389285..389584 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: JSA_RS01845
• Protein ID: WP_001060744.1
• Coordinates: 389073..389282 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSA_RS01800	384094..384924	-	831	Protein_337	KilA-N domain-containing protein	-
JSA_RS01810	385407..386624	-	1218	WP_000270122.1	site-specific integrase	-
JSA_RS01820	386918..387439	-	522	WP_001101554.1	hypothetical protein	-
JSA_RS01825	387565..388260	-	696	WP_000700230.1	helix-turn-helix domain-containing protein	-
JSA_RS01830	388405..388617	+	213	WP_000608987.1	hypothetical protein	-
JSA_RS01835	388650..388922	+	273	WP_000867969.1	hypothetical protein	-
JSA_RS01840	388934..389080	+	147	WP_000784888.1	hypothetical protein	-
JSA_RS01845	389073..389282	+	210	WP_001060744.1	hypothetical protein	Antitoxin
JSA_RS01850	389285..389584	+	300	WP_031904328.1	DUF1474 family protein	Toxin
JSA_RS01855	389659..391272	+	1614	WP_033854769.1	hypothetical protein	-
JSA_RS01860	391443..392246	+	804	WP_000148371.1	bifunctional DNA primase/polymerase	-
JSA_RS01865	392233..392505	+	273	WP_001149387.1	hypothetical protein	-
JSA_RS01870	392507..393156	+	650	Protein_349	pathogenicity island protein	-
JSA_RS01875	393495..393836	+	342	WP_001161659.1	hypothetical protein	-
JSA_RS01880	393848..394435	+	588	Protein_351	pathogenicity island protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	379052..404846	25794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11680.15 Da
• Isoelectric Point: 4.4740

>T30999 WP_031904328.1 NZ_AP014921:389285-389584 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKEKINDVAIKHGWFVEDKFVKNELETKQEHINFSASYLEHRIQNEHTVELLQVYLKEFGELIQKF
HEIEKASSDVSLATESDDA

Nucleotide

• Length: 300 bp

>T30999 NZ_AP014921:389285-389584 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCATAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8286.37 Da
• Isoelectric Point: 4.4976

>AT30999 WP_001060744.1 NZ_AP014921:389073-389282 [Staphylococcus aureus]
MNNKQKEVIEHVVYQLELSIMNNFESYEHTEYVDGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT30999 NZ_AP014921:389073-389282 [Staphylococcus aureus]
ATGAATAATAAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA