Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5656417..5657059 Replicon chromosome
Accession NZ_AP014864
Organism Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag KNN_RS29025 Protein ID WP_000635963.1
Coordinates 5656417..5656767 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KNN_RS29030 Protein ID WP_000004570.1
Coordinates 5656772..5657059 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN_RS29015 5653353..5653811 - 459 WP_000344234.1 SprT family protein -
KNN_RS36390 5654007..5654123 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KNN_RS29020 5654181..5656349 - 2169 WP_000426244.1 RNA-binding transcriptional accessory protein -
KNN_RS29025 5656417..5656767 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KNN_RS29030 5656772..5657059 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KNN_RS29035 5657368..5658537 - 1170 WP_000390615.1 alanine racemase -
KNN_RS29040 5658655..5659605 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
KNN_RS29045 5659762..5660121 - 360 WP_000583416.1 holo-ACP synthase -
KNN_RS29050 5660215..5660787 + 573 WP_000586917.1 rhomboid family intramembrane serine protease -
KNN_RS29055 5660780..5661742 - 963 WP_000961164.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T30995 WP_000635963.1 NZ_AP014864:c5656767-5656417 [Bacillus thuringiensis serovar tolworthi]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T30995 NZ_AP014864:c5656767-5656417 [Bacillus thuringiensis serovar tolworthi]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT30995 WP_000004570.1 NZ_AP014864:c5657059-5656772 [Bacillus thuringiensis serovar tolworthi]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT30995 NZ_AP014864:c5657059-5656772 [Bacillus thuringiensis serovar tolworthi]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References